Robinson Joshua F, Pennings Jeroen L A, Piersma Aldert H
National Institute for Public Health and the Environment-RIVM, Bilthoven, The Netherlands.
Methods Mol Biol. 2012;889:347-71. doi: 10.1007/978-1-61779-867-2_22.
Over the past decade, the use of gene expression profiling (i.e., toxicogenomics or transcriptomics) has been established as the vanguard "omics" technology to investigate exposure-induced molecular changes that underlie the development of disease. As this technology quickly advances, researchers are striving to keep pace in grasping the complexity of toxicogenomic response while at the same time determine its applicability for the field of developmental toxicology. Initial studies suggest toxicogenomics to be a promising tool for multiple types of study designs, including exposure-response investigations (dose and duration), chemical classification, and model comparisons. In this review, we examine the use of toxicogenomics in developmental toxicology, discussing biological and technical factors that influence response and interpretation. Additionally, we provide a framework to guide toxicogenomic investigations in the field of developmental toxicology.
在过去十年中，基因表达谱分析（即毒理基因组学或转录组学）已成为前沿的“组学”技术，用于研究暴露诱导的分子变化，这些变化是疾病发展的基础。随着这项技术的迅速发展，研究人员努力跟上步伐，掌握毒理基因组反应的复杂性，同时确定其在发育毒理学领域的适用性。初步研究表明，毒理基因组学是多种研究设计的有前途的工具，包括暴露-反应研究（剂量和持续时间）、化学分类和模型比较。在这篇综述中，我们研究了毒理基因组学在发育毒理学中的应用，讨论了影响反应和解释的生物学和技术因素。此外，我们提供了一个框架，以指导发育毒理学领域的毒理基因组学研究。
