PIWI蛋白可作为人类胃癌的预测标志物。
The PIWI protein acts as a predictive marker for human gastric cancer.
作者信息
Wang Yang, Liu Yanxia, Shen Xiaoying, Zhang Xiaoyan, Chen Ximei, Yang Changqin, Gao Hengjun
机构信息
Department of Gastroenterology, Institute of Digestive Diseases, Tongji Hospital Affliated to Tongji University, Shanghai 200065, China.
出版信息
Int J Clin Exp Pathol. 2012;5(4):315-25. Epub 2012 Apr 19.
PURPOSE
To investigate the expression of the human PIWI subfamily proteins in gastric cancer and their potential roles in the occurrence, development and prognosis of gastric cancer.
METHODS AND PATIENTS
Expression of the PIWI proteins were assessed by immunohistochemistry (IHC) in tissue microarrays (TMA), containing paired tumor tissue and adjacent non-cancer tissue from 182 patients who had undergone surgery in hospital for histologically proven gastric cancer (GC). Prognostic value and correlation with other clinicopathologic factors were evaluated in two classifications.
RESULTS
The expression of PIWIL1-4 was significantly higher in tumor tissue than that in adjacent tissue; A significant correlation was observed between the higher expression of PIWI protein with the T stage, lymph node metastasis and clinical TNM (cTNM); Survival analysis by Kaplan-Meier survival curve and log-rank test demonstrated that elevated PIWIL1 and PIWIL2 expression in cancer tissue predicted poorer overall survival (OS) compared with group in lower expression (36.5% VS 67.6%; 37.4% VS 54.2%; respectively). Notably, multivariate analyses by Cox's proportional hazard model revealed that expression of PIWIL1 was an independent prognostic factor in gastric cancer.
CONCLUSIONS
The PIWI subfamily protein is an absolutely key molecular factor along with the tumor occurrence and development. And the PIWI protein could act as a potential biomarker for prognosis evaluation of gastric cancer.
目的
研究人类PIWI亚家族蛋白在胃癌中的表达及其在胃癌发生、发展和预后中的潜在作用。
方法与患者
采用免疫组织化学（IHC）方法，在组织芯片（TMA）中评估PIWI蛋白的表达，该组织芯片包含182例经组织学证实为胃癌（GC）且在我院接受手术的患者的配对肿瘤组织和相邻非癌组织。在两种分类中评估其预后价值以及与其他临床病理因素的相关性。
结果
PIWIL1 - 4在肿瘤组织中的表达显著高于相邻组织；PIWI蛋白的高表达与T分期、淋巴结转移及临床TNM（cTNM）之间存在显著相关性；通过Kaplan - Meier生存曲线和对数秩检验进行的生存分析表明，与低表达组相比，癌组织中PIWIL1和PIWIL2表达升高预示总体生存期（OS）较差（分别为36.5%对67.6%；37.4%对54.2%）。值得注意的是，通过Cox比例风险模型进行的多因素分析显示，PIWIL1的表达是胃癌的独立预后因素。
结论
PIWI亚家族蛋白是肿瘤发生和发展过程中绝对关键的分子因素。并且PIWI蛋白可作为评估胃癌预后的潜在生物标志物。
Zotero 插件