Wang Jue, Shen Hongchang, Fu Guobin, Zhao Dandan, Wang Weibo
Department of Breast and Thyroid Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China.
Department of Chemotherapy, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China.
Oncotarget. 2017 Feb 7;8(6):9442-9450. doi: 10.18632/oncotarget.14217.
We have performed this retrospective study to elucidate whether elevated expression of the overexpressed in lung cancer 1 (OLC1) was related to the clinicopathological parameters and prognosis of patients with gastric adenocarcinoma. Additionally, different effects of various subcellular OLC1 expression on gastric adeno-carcinogenesis were focused on in our study. Both overall and subcellular expression of OLC1 was evaluated by immunohistochemistry(IHC) via tissue microarrays from total 393 samples. The Kaplan-Meier method and Cox's proportional hazard model were exerted to further explore the correlation between OLC1 and prognosis. Total overexpression of OLC1 was significantly associated with stage (P = 0.004) and differentiation (P = 0.009), and only the strong total expression could predict a poor prognosis (HR = 1.31, P = 0.04). There were significant associations found between nuclear overexpression and tumor invasion depth(P = 0.002), lymph node (P < 0.001), stage (P = 0.004), differentiation (P < 0.001) and smoking history (P = 0.045). Furthermore, over-expressed nuclear OLC1 protein could be an independent risk factor for gastric adenocarcinoma (univariate: HR = 1.43, P = 0.003; multivariate: HR = 1.39, P = 0.011). In general, both total and nuclear overexpression of OLC1 could be the signs of gastric adeno-carcinogenesis, which might be served as the biomarkers for diagnosis at an early stage, even at the onset of tumorigenesis. Rather than the total expression, nuclear overexpression of OLC1 was correlated with most clinicopathological parameters and could predict a poor overall survival as an independent factor for prognosis, which made it a more effective and sensitive biomarker for gastric adenocarcinoma.
我们进行了这项回顾性研究，以阐明肺癌中过表达1（OLC1）的表达升高是否与胃腺癌患者的临床病理参数及预后相关。此外，本研究还关注了OLC1在不同亚细胞中的表达对胃腺癌发生的不同影响。通过对总共393个样本的组织微阵列进行免疫组织化学（IHC）评估OLC1的整体和亚细胞表达。采用Kaplan-Meier法和Cox比例风险模型进一步探讨OLC1与预后的相关性。OLC1的整体过表达与分期（P = 0.004）和分化程度（P = 0.009）显著相关，只有强烈的整体表达可预测预后不良（HR = 1.31，P = 0.04）。细胞核过表达与肿瘤浸润深度（P = 0.002）、淋巴结（P < 0.001）、分期（P = 0.004）、分化程度（P < 0.001）及吸烟史（P = 0.045）之间存在显著关联。此外，过表达的细胞核OLC1蛋白可能是胃腺癌的独立危险因素（单因素：HR = 1.43，P = 0.003；多因素：HR = 1.39，P = 0.011）。总体而言，OLC1的整体和细胞核过表达均可能是胃腺癌发生的标志，可作为早期诊断甚至肿瘤发生起始阶段的生物标志物。与整体表达相比，OLC1的细胞核过表达与大多数临床病理参数相关，并可作为独立的预后因素预测较差的总生存期，这使其成为胃腺癌更有效、更敏感的生物标志物。
