Inst of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ of Belgrade, Serbia.
J Food Sci. 2012 Jul;77(7):C779-83. doi: 10.1111/j.1750-3841.2012.02756.x. Epub 2012 Jun 4.
On the basis of LC/UV/MS analysis, 35 compounds from the extracts of seeds of black, red, and white currants (2 cultivars of each) were identified. Black currants cultivars contained protocatechuic acid and p-hydroxybenzoic acid, and traces of nitril containing phenolic acids. The presence of synapoyl glucoside was characteristic for cv. Malling Jewel. Sesquiterpenoid glycosides and carboxymethylindol glycosides were present mainly in white and red currant cultivars. Blackcurrant seeds contained higher amounts of flavonoids, especially rutin, isoquercetin, and taxifolin. The currant seed extracts were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The frequency of MN was scored in binucleated cells, and the cultivars of black currants showed the best antioxidant potential. At a concentration of only 0.17 mg/mL, extract of the seed cv. Malling Jewel effected a decrease in the frequency of MN of 60% compared with control cell cultures.
Our results provide evidence of protective effects of currant seed extracts and isolated pure compounds on cytogenetic damages in human lymphocytes. Thus, currant seed extracts could exert beneficial effects in quite a few diseases, for many of the biological actions have been attributed to their antioxidant properties.
根据 LC/UV/MS 分析，从黑、红、白三种醋栗（每种两个品种）种子提取物中鉴定出 35 种化合物。黑醋栗品种含有原儿茶酸和对羟基苯甲酸，以及痕量含腈基的酚酸。Malling Jewel 品种的特征是含有咖啡酰奎宁酸葡萄糖苷。倍半萜糖苷和羧甲基吲哚糖苷主要存在于白醋栗和红醋栗品种中。黑醋栗种子含有更高含量的类黄酮，特别是芦丁、异槲皮苷和杨梅素。使用细胞松弛素 B 阻断微核（CBMN）试验，对醋栗种子提取物的体外保护染色体畸变的作用进行了测试。在双核细胞中对 MN 的频率进行评分，黑醋栗品种显示出最好的抗氧化潜力。在浓度仅为 0.17mg/ml 时，与对照细胞培养物相比，Malling Jewel 品种种子提取物可使 MN 的频率降低 60%。
我们的研究结果提供了醋栗种子提取物和分离的纯化合物对人淋巴细胞细胞遗传学损伤的保护作用的证据。因此，醋栗种子提取物可能对许多疾病有有益的作用，因为许多生物作用归因于其抗氧化特性。
