Mortimer J T, Yodlowski E H
J Med Eng Technol. 1979 Sep;3(5):242-7. doi: 10.3109/03091907909160663.
A new technique for measuring neuromuscular junction continuity is described. Junctional transmission is evaluated by integration of the electromyographic (EMG) response induced by intramuscular electrical stimulation. The unique characteristics of this monitoring technique are the use of intramuscular stimulation and a varying stimulation rate. The stimulus frequency is made to increase exponentially from 1 to 100 pulses per second over a period of 10 or 15 seconds. Thin coiled wire electrodes are used for stimulation and recording of the EMG response. The frequency sweep electromyogram (FS-EMB) response was measured in uncurarized and curarized tibialis anterior muscle of cat. In the uncurarized preparation, the FS-EMG response was only slightly decreased with an increasing stimulus frequency. During neuromuscular blockade with d-tubocurarine the FS-EMG response diminished with increasing frequency. The response failed at successively lower frequencies during deeper levels of neuromuscular block. Recovery proceeded in the reverse order. The frequency sweep technique provides quantitative assessment of neuromuscular junction continuity over a broad spectrum of driving frequencies without inducing fatigue. This method does not require force measurements nor voluntary effort. Intramuscular stimulation minimises painful stimuli thus making the frequency sweep technique suitable for evaluating neuromuscular transmission in either conscious or unconscious individuals.
本文描述了一种测量神经肌肉接头连续性的新技术。通过整合肌内电刺激诱发的肌电图（EMG）反应来评估接头传递。这种监测技术的独特之处在于使用肌内刺激和变化的刺激速率。刺激频率在10或15秒内从每秒1次脉冲呈指数增加至每秒100次脉冲。细线圈状电极用于刺激和记录EMG反应。在未用箭毒和用箭毒处理的猫胫前肌中测量频率扫描肌电图（FS-EMB）反应。在未用箭毒处理的标本中，随着刺激频率增加，FS-EMG反应仅略有下降。在用d-筒箭毒碱进行神经肌肉阻滞期间，FS-EMG反应随频率增加而减弱。在更深程度的神经肌肉阻滞时，反应在逐渐降低的频率下失败。恢复则按相反顺序进行。频率扫描技术可在不引起疲劳的情况下，在广泛的驱动频率范围内对接头连续性进行定量评估。该方法不需要测量力量，也不需要受试者自愿用力。肌内刺激将疼痛刺激降至最低，因此频率扫描技术适用于评估清醒或无意识个体的神经肌肉传递。
