Zhao Y H, Krueger J G, Sudol M
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.
Oncogene. 1990 Nov;5(11):1629-35.
Expression of the c-yes proto-oncogene in normal mammalian tissues was studied by immunohistochemistry and by immunoprecipitation assays using affinity purified anti-yes IgG. The antibody was raised against the amino-terminal domain of the human c-yes protein expressed in bacteria as a trpE-yes fusion protein. We detected p62c-yes in a variety of rat and human tissues but its relative level of expression varied significantly among different cell types. Certain epithelial cells, platelets and spermatid acrosomes showed the highest levels of p62c-yes. Intracellular localization of p62c-yes in epithelial cells differed among organs performing different physiological functions. The overall pattern of expression suggests that p62c-yes may be involved in numerous cellular pathways.
通过免疫组织化学以及使用亲和纯化的抗yes IgG进行免疫沉淀分析，研究了原癌基因c-yes在正常哺乳动物组织中的表达情况。该抗体是针对在细菌中作为trpE-yes融合蛋白表达的人c-yes蛋白的氨基末端结构域产生的。我们在多种大鼠和人类组织中检测到了p62c-yes，但其相对表达水平在不同细胞类型之间存在显著差异。某些上皮细胞、血小板和精子细胞顶体显示出最高水平的p62c-yes。在执行不同生理功能的器官中，上皮细胞中p62c-yes的细胞内定位有所不同。整体表达模式表明p62c-yes可能参与众多细胞途径。
