Shaulsky G, Ben-Ze'ev A, Rotter V
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Oncogene. 1990 Nov;5(11):1707-11.
The expression of p53, a transformation associated protein, has been found to be regulated during the cell cycle. We show here that the subcellular localization of p53 varies throughout the cell cycle. In growth stimulated Balb/c 3T3 cells, p53 is produced at elevated levels and the newly synthesized protein accumulates in the cytoplasm during the G1 phase. Around the beginning of the S phase, p53 accumulates in the cell nucleus, where it stays for about 3 h. Following this initial step of DNA synthesis, p53 is no longer found in the nuclear compartment, but rather accumulates in the cytoplasm. This modulation in the subcellular localization of p53 suggests that the protein is spatially regulated during cell cycle.
已发现一种与转化相关的蛋白质p53的表达在细胞周期中受到调控。我们在此表明，p53的亚细胞定位在整个细胞周期中有所不同。在生长受刺激的Balb/c 3T3细胞中，p53的产生水平升高，新合成的蛋白质在G1期积聚在细胞质中。在S期开始前后，p53积聚在细胞核中，并在那里停留约3小时。在DNA合成的这一初始步骤之后，p53不再存在于核区室中，而是积聚在细胞质中。p53亚细胞定位的这种调节表明该蛋白质在细胞周期中受到空间调控。
