MMP3 和 TIMP1 变体与慢性牙周炎有关，可能与疾病进展有关。
MMP3 and TIMP1 variants contribute to chronic periodontitis and may be implicated in disease progression.
机构信息
Department of Endodontics, School of Dentistry, and Pediatric Research Center, Medical School, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
出版信息
J Clin Periodontol. 2012 Aug;39(8):707-16. doi: 10.1111/j.1600-051X.2012.01902.x. Epub 2012 Jun 3.
AIM
Matrix metalloproteinases (MMPs) play a key role in the tissue destruction characteristic of chronic periodontitis. The purpose of this study was to investigate the association of MMP and TIMP polymorphisms with chronic periodontitis in two populations.
MATERIAL AND METHODS
A total of 34 polymorphisms spanning 12 MMP and 2 TIMP genes were genotyped in 401 individuals from Brazil (99 cases with chronic periodontitis and 302 controls), and 274 individuals from the US (70 cases and 204 controls). Individuals were considered cases if presenting at least three teeth exhibiting sites of clinical attachment loss ≥ 5 mm in two different quadrants. Controls were characterized by absence of clinical attachment loss and no sites with probing depth >3 mm. MMP3 and TIMP1 mRNA expression was evaluated in healthy and diseased periodontal tissues.
RESULTS
TIMP1 showed association with chronic periodontitis in the Brazilian population (for rs5906435, p = 0.0004), whereas MMP3 showed association in the US population (for rs679620, p = 0.0003; and rs650108, p = 0.002) and in the Brazilian population (for rs639752, p = 0.005). MMP3 and TIMP1 mRNA expression was significantly higher in diseased tissues when compared to control tissues.
CONCLUSIONS
Our results further support a role for variations in MMP3 in chronic periodontitis and report a novel association with TIMP1. These genes may be considered additional candidate genes for chronic periodontitis.
目的
基质金属蛋白酶（MMPs）在慢性牙周炎的组织破坏中起着关键作用。本研究旨在调查 MMP 和 TIMP 多态性与两个人群慢性牙周炎的关系。
材料和方法
在巴西的 401 名个体（99 例慢性牙周炎患者和 302 名对照者）和美国的 274 名个体（70 例患者和 204 名对照者）中，共检测了 12 个 MMP 和 2 个 TIMP 基因的 34 个多态性。如果至少有三个牙齿在两个不同象限的三个位点出现临床附着丧失≥5mm，则将个体视为病例。对照者的特征是无临床附着丧失，且无探诊深度>3mm 的位点。在健康和患病牙周组织中评估了 MMP3 和 TIMP1 的 mRNA 表达。
结果
TIMP1 在巴西人群中与慢性牙周炎相关（rs5906435，p=0.0004），而 MMP3 在美人群中与慢性牙周炎相关（rs679620，p=0.0003；rs650108，p=0.002）和巴西人群中相关（rs639752，p=0.005）。与对照组织相比，患病组织中 MMP3 和 TIMP1 的 mRNA 表达显著升高。
结论
我们的结果进一步支持 MMP3 变异在慢性牙周炎中的作用，并报告了与 TIMP1 的新关联。这些基因可被视为慢性牙周炎的附加候选基因。
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