Curtin Health Innovation Research Institute, School of Pharmacy, Curtin University, Perth, WA, Australia.
Skin Res Technol. 2013 Feb;19(1):e283-9. doi: 10.1111/j.1600-0846.2012.00640.x. Epub 2012 Jun 7.
BACKGROUND/AIMS: 5-Aminolevulinate (ALA) is an important photodynamic therapy drug for the treatment of actinic keratoses and other non-melanoma skin cancers in cosmetically sensitive areas. One limitation of this drug is a relatively high recurrence rate. Our aim was to evaluate the feasibility of ultrasound augmented ALA delivery in excised human skin.
An ultrasonic delivery device was used to enhance radiolabelled ALA into excised skin. Quantification of ALA was performed after passive and ultrasonic ALA delivery. Transepidermal water loss was used as a measure of barrier function before and after ultrasonic treatment.
We found that ultrasonic treatment dramatically increased the mean cumulative amount of ALA to P< 0.0001 from 4 to 8 h when compared to passive ALA treatment. The flux was calculated to be 54.8 ± 8.0 μg/cm(2) h with ultrasound treatment. TEWL increased nearly two-fold, from 12.3 to 21.0, after ultrasound treatment.
Our study supports the use of ultrasound for improved ALA delivery by showing significant improvements in the cumulative drug load and flux via combined ultrasound and ALA treatment.
背景/目的：5-氨基酮戊酸（ALA）是一种重要的光动力治疗药物，可用于治疗美容敏感部位的光化性角化病和其他非黑素瘤皮肤癌。该药物的一个局限性是相对较高的复发率。我们的目的是评估超声增强ALA 在离体人皮肤上传递的可行性。
使用超声传递装置将放射性标记的 ALA 传递到离体皮肤中。在被动和超声 ALA 传递后进行 ALA 的定量。经皮水分流失（TEWL）用于在超声处理前后测量屏障功能。
与被动 ALA 处理相比，我们发现超声处理可使平均累积 ALA 量从 4 小时增加到 8 小时，差异具有统计学意义（P<0.0001）。超声处理时的通量计算为 54.8±8.0μg/cm²·h。TEWL 在超声处理后增加了近两倍，从 12.3 增加到 21.0。
我们的研究支持使用超声来改善 ALA 的传递，因为联合超声和 ALA 治疗可显著提高累积药物负荷和通量。
