Choi J H, Shin E J, Jeong K H, Shin M K
Department of Dermatology, College of Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemun-Ku, Seoul, 130-702, South Korea.
Lasers Med Sci. 2017 Nov;32(8):1895-1900. doi: 10.1007/s10103-017-2305-8. Epub 2017 Aug 19.
Successful delivery of a photosensitizer into the skin is an important factor for effective photodynamic therapy (PDT). The effective method to increase drug penetration within short incubation time overcoming skin barrier have been investigated. This study was performed to analyze and compare the effectiveness of ablative fractional laser (FXL) pretreatment and/or sonophoresis for enhancing the penetration of 5-aminolevulinic acid (ALA) into human skin in vivo. Twenty-four identical 1 × 1 cm treatment areas were mapped on the backs of ten healthy male subjects. Each area received FXL pretreatment and/or sonophoresis with different energy settings and ALA incubation times. After treatments, porphyrin fluorescence reflecting the ALA penetration were measured. Application of ablative CO FXL pretreatment resulted to higher fluorescence intensities than the non-treatment group. Incubation times were positively correlated with the increments of ALA penetration. However, increasing pulse energy or combining with sonophoresis did not show additional positive effects on ALA penetration. Ablative CO FXL pretreatment effectively facilitated ALA penetration in human skin in vivo. Ablative CO FXL alone without sonophoresis setting pulse energy of 10 and 20 mJ with more than 60 min of ALA incubation time could be an ideal setting for ALA penetration.
将光敏剂成功递送至皮肤是有效进行光动力疗法（PDT）的一个重要因素。人们已经研究了在短孵育时间内克服皮肤屏障以增加药物渗透的有效方法。本研究旨在分析和比较剥脱性点阵激光（FXL）预处理和/或超声导入法在增强5-氨基乙酰丙酸（ALA）体内渗透至人皮肤方面的有效性。在十名健康男性受试者的背部绘制了24个相同的1×1平方厘米的治疗区域。每个区域接受不同能量设置和ALA孵育时间的FXL预处理和/或超声导入法。治疗后，测量反映ALA渗透情况的卟啉荧光。剥脱性CO2 FXL预处理组的荧光强度高于未治疗组。孵育时间与ALA渗透的增加呈正相关。然而，增加脉冲能量或与超声导入法联合使用对ALA渗透并未显示出额外的积极效果。剥脱性CO2 FXL预处理有效地促进了ALA在人皮肤中的体内渗透。单独使用剥脱性CO2 FXL且不进行超声导入，设置10和20 mJ的脉冲能量以及超过60分钟的ALA孵育时间可能是ALA渗透的理想设置。
