人类肺癌 microRNA 表达谱研究的荟萃分析，比较癌症组织与正常组织。
Meta-analysis of human lung cancer microRNA expression profiling studies comparing cancer tissues with normal tissues.
机构信息
Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110001, China.
出版信息
J Exp Clin Cancer Res. 2012 Jun 6;31(1):54. doi: 10.1186/1756-9966-31-54.
BACKGROUND
Lung cancer is the major cause of cancer death globally, it is often diagnosed at an advanced stage and has one of the lowest survival rates of any type of cancer. The common interest in the field of lung cancer research is the identification of biomarkers for early diagnosis and accurate prognosis. There is increasing evidence to suggest that microRNAs play important and complex roles in lung cancer.
METHODS
A meta-analysis was conducted to review the published microRNA expression profiling studies that compared the microRNAs expression profiles in lung cancer tissues with those in normal lung tissues. A vote-counting strategy that considers the total number of studies reporting its differential expression, the total number of tissue samples used in the studies and the average fold change was employed.
RESULTS
A total of 184 differentially expressed microRNAs were reported in the fourteen microRNA expression profiling studies that compared lung cancer tissues with normal tissues, with 61 microRNAs were reported in at least two studies. In the panel of consistently reported up-regulated microRNAs, miR-210 was reported in nine studies and miR-21 was reported in seven studies. In the consistently reported down-regulated microRNAs, miR-126 was reported in ten studies and miR-30a was reported in eight studies. Four up-regulated microRNAs (miR-210, miR-21, miR-31 and miR-182) and two down-regulated mcroiRNAs (miR-126 and miR-145) were consistently reported both in squamous carcinoma and adenocarcinoma-based subgroup analysis, with the other 14 microRNAs solely reported in one or the other subset.
CONCLUSIONS
In conclusion, the top two most consistently reported up-regulated microRNAs were miR-210 and miR-21. The results of this meta-analysis of human lung cancer microRNA expression profiling studies might provide some clues of the potential biomarkers in lung cancer. Further mechanistic and external validation studies are needed for their clinical significance and role in the development of lung cancer.
背景
肺癌是全球癌症死亡的主要原因，通常在晚期诊断，并且是所有癌症类型中存活率最低的之一。肺癌研究领域的共同关注点是确定用于早期诊断和准确预后的生物标志物。越来越多的证据表明，microRNAs 在肺癌中发挥着重要而复杂的作用。
方法
进行了荟萃分析，以回顾比较肺癌组织与正常肺组织中 microRNA 表达谱的已发表的 microRNA 表达谱研究。采用一种计票策略，该策略考虑了报告其差异表达的研究总数、研究中使用的组织样本总数和平均倍数变化。
结果
在比较肺癌组织与正常组织的十四项 microRNA 表达谱研究中，共报道了 184 个差异表达的 microRNAs，其中 61 个 microRNAs在至少两项研究中报道。在一致报道上调的 microRNAs 中，miR-210 在 9 项研究中报道，miR-21 在 7 项研究中报道。在一致报道下调的 microRNAs 中，miR-126 在 10 项研究中报道，miR-30a 在 8 项研究中报道。四个上调的 microRNAs（miR-210、miR-21、miR-31 和 miR-182）和两个下调的 microRNAs（miR-126 和 miR-145）在鳞状细胞癌和腺癌亚组分析中均一致报道，而其他 14 个 microRNAs 仅在一个或另一个亚组中报道。
结论
总之，最一致报道的两个上调 microRNAs 是 miR-210 和 miR-21。这项对人类肺癌 microRNA 表达谱研究的荟萃分析结果可能为肺癌潜在生物标志物提供了一些线索。需要进一步进行机制和外部验证研究，以确定它们在肺癌中的临床意义和作用。
Zotero 插件