实验性颈椎病脊髓病中 NF-κB/p50、NF-κB/p65、MMP-9、MMP-2 和 u-PA 的免疫组织化学特征。

Immunohistochemical profile of NF-κB/p50, NF-κB/p65, MMP-9, MMP-2, and u-PA in experimental cervical spondylotic myelopathy.

机构信息

Department of Neurosurgery, University Hospital of Patras, Rio Patras, Greece.

出版信息

Spine (Phila Pa 1976). 2013 Jan 1;38(1):4-10. doi: 10.1097/BRS.0b013e318261ea6f.

DOI:10.1097/BRS.0b013e318261ea6f

PMID:22673181

Abstract

STUDY DESIGN

The immunohistochemical profile of nuclear factor-κ B (NF-κB)/p50, NF-κB/p65, matrix metalloproteinase (MMP)-9, MMP-2, and urokinase-type plasminogen activator (u-PA) proteins was examined in spinal cord tissues coming from rabbits, which underwent chronic cervical spinal cord compression.

OBJECTIVE

To study the potential role of NF-κB and extracellular matrix proteins under the chronic mechanical compression of the cervical spinal cord.

SUMMARY OF BACKGROUND DATA

Cervical spondylotic myelopathy (CSM) is the most common cause of spinal cord dysfunction among adults older than 55 years. Neuronal loss, myelin destruction, axonal degeneration, and glial scar formation are the principal neuropathological features of CSM. However, the biologic pathways that lead to these features remain unclear.

METHODS

In this study, we used a new animal experimental model of CSM developed in our laboratory. Briefly, after posterior cervical laminectomy, gradual and progressive compression (during 20 weeks) was achieved by introducing a piece of aromatic polyether (0.07 mm thick) under the C6 lamina in 15 New Zealand rabbits. In control animals (n = 15), the aromatic polyether was implanted and then removed after 60 seconds (sham operation). The immunoreactivity of p50 and p65 subunits of NF-kB, as well as that of MMP-2, MMP-9, and u-PA, was evaluated in paraffin-embedded spinal cord sections coming from both groups. The evaluation was performed using immunohistochemistry technique and the results were analyzed using SPSS for Windows, release 12.0 (SPSS Inc., Chicago, IL).

RESULTS

Increased immunoreactivity of both NF-κB subunits, p50 and p65, as well as MMP-2, MMP-9, and u-PA was demonstrated in animals with CSM in comparison with controls. Statistical analysis of the results revealed strong positive correlation between NF-κB subunits immunoreactivity and that of MMP-9, MMP-2, and u-PA.

CONCLUSION

There is a strong correlation between the immunoexpression of NF-κB/p50, NF-κB/p65, MMP-2, MMP-9, u-PA, and CSM.

摘要

研究设计

本研究通过对慢性颈脊髓压迫模型兔脊髓组织进行核因子-κ B（NF-κ B）/p50、NF-κ B/p65、基质金属蛋白酶（MMP）-9、MMP-2 和尿激酶型纤溶酶原激活物（u-PA）蛋白的免疫组化分析，探讨 NF-κ B 及细胞外基质蛋白在慢性颈脊髓机械压迫下的潜在作用。

目的

研究 NF-κ B 及其细胞外基质蛋白在慢性颈脊髓压迫下的潜在作用。

背景资料概述

颈椎病是 55 岁以上成年人脊髓功能障碍的最常见原因。神经元丢失、髓鞘破坏、轴突变性和胶质瘢痕形成是颈椎病的主要神经病理学特征。但是，导致这些特征的生物学途径仍不清楚。

方法

本研究采用我们实验室建立的新型颈椎病动物实验模型。简而言之，在颈后路椎板切除术后，在 15 只新西兰兔的 C6 椎板下置入一片芳香聚醚（厚 0.07mm），逐渐造成进行性压迫（20 周）。在对照组动物（n=15）中，芳香聚醚植入 60 秒后取出（假手术）。采用免疫组化技术检测两组石蜡包埋脊髓切片中 p50 和 p65 亚单位 NF-κ B、MMP-2、MMP-9 和 u-PA 的免疫反应性，并使用 SPSS for Windows，release 12.0（SPSS Inc.，Chicago，IL）进行分析。