Cariology and Operative Dentistry, Department of Restorative Science, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Dent Mater J. 2012;31(3):362-7. doi: 10.4012/dmj.2011-203. Epub 2012 May 14.
The aims of this study were to investigate the effects of hesperidin, a citrus flavonoid, on human root dentin demineralization and collagen preservation, and compare it with chlorhexidine and grape seed extract. Specimens were assigned to different treatment groups: hesperidin, chlorhexidine and grape seed extract. Specimens were subjected to pH cycling by demineralization for 14 h, incubation in testing solutions for 2 h and remineralization in presence of bacterial-derived collagenase for 8 h, for 8 days. Calcium release was measured by means of an atomic absorption spectrophotometer, and degraded collagen matrix was investigated by hydroxyproline assay. Specimens were assessed longitudinally with transverse micro-radiography to investigate lesion depth and mineral loss. In hesperidin and grape seed extract groups, demineralization was reduced when the collagen matrix was preserved. The hesperidin group showed the lowest value in lesion depth and mineral loss, indicating that hesperidin inhibited demineralization and probably enhanced remineralization even under fluoride-free conditions.
本研究旨在探讨桔皮苷（一种柑橘类黄酮）对人牙根脱矿和胶原保存的影响，并将其与洗必泰及葡萄籽提取物进行比较。标本被分为不同的处理组：桔皮苷、洗必泰和葡萄籽提取物。标本经过 14 小时的脱矿、2 小时的测试溶液孵育以及 8 小时的细菌来源胶原酶再矿化处理，进行 8 天的 pH 值循环。通过原子吸收分光光度计测量钙释放，通过羟脯氨酸测定法研究降解的胶原基质。标本通过横向微放射摄影进行纵向评估，以研究病变深度和矿物质损失。在桔皮苷和葡萄籽提取物组中，当胶原基质得以保存时，脱矿作用减少。桔皮苷组的病变深度和矿物质损失最低，表明桔皮苷抑制脱矿作用，甚至在无氟条件下可能增强再矿化作用。
