Restrepo Berta Nelly, Arboleda Margarita, Ramirez Ruth, Alvarez Gonzalo
Instituto Colombiano de Medicina Tropical-Universidad CES, Medellín, Colombia.
Biomedica. 2011 Oct-Dec;31(4):599-607. doi: 10.1590/S0120-41572011000400015.
Severe dengue infection is characterized by enhanced vascular permeability produced by cytokines and biochemical mediators. One of these mediators is the platelet-activating factor. Degradation of platelet-activating factor is accomplished by its specific acetylhydrolase. Down or up regulation of this enzyme has been linked to several diseases. However, the role of this enzyme in dengue infection is unknown, a well as whether differential activity occurs by ethnic group.
The activity of the platelet-activating factor-acetylhydrolase was compared in patients infected with dengue virus in two ethnic groups.
A descriptive, prospective and longitudinal study was made in two states of Colombia (Antioquia and Chocó). Serum samples were taken from 43 mestizo patients and 33 patients of African descent, each taken during five consecutive days of the acute dengue phase. A second serum sample was taken during the convalescent phase.
The mestizo patients showed higher frequency of dengue hemorrhagic fever than the patients of African descent (23.3% vs. 12.1%, p=0.25). The serum activity of the platelet-activating factor acetylhydrolase was higher in African descendents than in mestizos (0.89, CI: 0.72-1.10 vs. 0.76 CI: 0-1.03, p<0.001). This relationship is maintained for occurence of dengue fever (0.89, CI: 0.73–1.10 vs. 0.73, CI: 0–1.05, p<0.001), but not significant for dengue hemorrhagic fever (0.88, CI: 0.69–1.12 vs. 0.83, CI: 0.71–1.08, p=0.89). CONCLUSION. An increased production of platelet-activating factor-acetylhydrolase occurs in the serum of dengue-infected African descendants. However, a study of the frequencies of polymorphisms for this enzyme will permit more conclusive support for these observations.
严重登革热感染的特征是细胞因子和生化介质导致血管通透性增强。其中一种介质是血小板活化因子。血小板活化因子的降解是由其特异性乙酰水解酶完成的。这种酶的下调或上调与多种疾病有关。然而，这种酶在登革热感染中的作用尚不清楚，不同种族之间是否存在差异活性也不清楚。
比较两个种族登革病毒感染患者中血小板活化因子乙酰水解酶的活性。
在哥伦比亚的两个州（安蒂奥基亚和乔科）进行了一项描述性、前瞻性和纵向研究。从43名混血患者和33名非洲裔患者中采集血清样本，在急性登革热阶段连续五天每天采集一次。在恢复期采集第二份血清样本。
混血患者登革出血热的发生率高于非洲裔患者（23.3%对12.1%，p = 0.25）。非洲裔患者血清中血小板活化因子乙酰水解酶的活性高于混血患者（0.89，可信区间：0.72 - 1.10对0.76，可信区间：0 - 1.03，p < 0.001）。登革热发生时这种关系依然存在（0.89，可信区间：0.73–1.10对0.73，可信区间：0–1.05，p < 0.001），但登革出血热时无显著差异（0.88，可信区间：0.69–1.12对0.83，可信区间：0.71–1.08，p = 0.89）。结论：登革热感染的非洲裔患者血清中血小板活化因子乙酰水解酶的产生增加。然而，对该酶多态性频率的研究将为这些观察结果提供更确凿的支持。
