Gale Trevor V, Horton Timothy M, Grant Donald S, Garry Robert F
Department of Microbiology and Immunology, Tulane University, New Orleans, Louisiana, United States of America.
Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone.
PLoS Negl Trop Dis. 2017 Sep 18;11(9):e0005943. doi: 10.1371/journal.pntd.0005943. eCollection 2017 Sep.
Lassa fever afflicts tens of thousands of people in West Africa annually. The rapid progression of patients from febrile illness to fulminant syndrome and death provides incentive for development of clinical prognostic markers that can guide case management. The small molecule profile of serum from febrile patients triaged to the Viral Hemorrhagic Fever Ward at Kenema Government Hospital in Sierra Leone was assessed using untargeted Ultra High Performance Liquid Chromatography Mass Spectrometry. Physiological dysregulation resulting from Lassa virus (LASV) infection occurs at the small molecule level. Effects of LASV infection on pathways mediating blood coagulation, and lipid, amino acid, nucleic acid metabolism are manifest in changes in the levels of numerous metabolites in the circulation. Several compounds, including platelet activating factor (PAF), PAF-like molecules and products of heme breakdown emerged as candidates that may prove useful in diagnostic assays to inform better care of Lassa fever patients.
拉沙热每年在西非折磨着数万人。患者从发热性疾病迅速发展为暴发性综合征并死亡，这促使人们开发能够指导病例管理的临床预后标志物。利用非靶向超高效液相色谱质谱法评估了在塞拉利昂凯内马政府医院被分诊到病毒性出血热病房的发热患者血清中的小分子谱。拉沙病毒（LASV）感染导致的生理失调发生在小分子水平。LASV感染对介导血液凝固以及脂质、氨基酸、核酸代谢的途径的影响表现为循环中多种代谢物水平的变化。包括血小板活化因子（PAF）、PAF样分子和血红素分解产物在内的几种化合物成为了可能在诊断检测中有用的候选物，有助于更好地治疗拉沙热患者。
