Coutinho Henrique D M, Matias Edinardo F F, Santos Karla K A, Santos Francisco A V, Morais-Braga Maria Flaviana B, Souza Teógenes M, Andrade Jacqueline Cosmo, Souza Celestina E S, Tintino Saulo R, Guedes Gláucia M M, Falcão-Silva Vivyanne S, Siqueira-Júnior José P, Costa José G M
Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato, CE, Brasil.
Biomedica. 2011 Oct-Dec;31(4):608-12. doi: 10.1590/S0120-41572011000400016.
Some species of Staphylococcus are often recognized as etiological agents of many animal and human opportunistic infections. This study is the first test of change in resistance of antibiotic activity by Croton campestris A. and Ocimum gratissimum L. against multiresistant strains of Staphylococcus aureus.
In this study, the hexane and methanol extract of Croton campestris A. and Ocimum gratissimum L. was tested for antibacterial activity alone and in combination with norfloxacin against the strain SA1199B.
The minimum inhibitory concentration (MIC) and the modulatory effect of extracts was assayed using microtitre assay.
By the fact of the MIC observed was not clinically relevant (MIC= 512 to ≥1.024 µg/ml), the antibiotic activity of norfloxacin was enhanced when this antibiotic was combined with sub-inhibitory concentrations of extracts, mainly the hexane extracts.
These results indicate that the assayed extracts present compounds that can be used as a putative efflux pump inhibitor, indicating that Croton campestris A. and Ocimum gratissimum L. can be a source of plant derived products with antibiotic modifier activity.
某些葡萄球菌属物种常被认为是许多动物和人类机会性感染的病原体。本研究首次测试了原野巴豆和丁香罗勒对耐多药金黄色葡萄球菌菌株的抗生素活性抗性变化。
在本研究中，测试了原野巴豆和丁香罗勒的己烷提取物和甲醇提取物单独以及与诺氟沙星联合对SA1199B菌株的抗菌活性。
使用微量滴定法测定提取物的最低抑菌浓度（MIC）和调节作用。
鉴于观察到的MIC在临床上不相关（MIC = 512至≥1.024μg/ml），当该抗生素与亚抑制浓度的提取物（主要是己烷提取物）联合使用时，诺氟沙星的抗生素活性增强。
这些结果表明，所测定的提取物含有可作为推定的外排泵抑制剂的化合物，这表明原野巴豆和丁香罗勒可能是具有抗生素修饰活性的植物源产品的来源。
