巴豆和罗勒对金黄色葡萄球菌诺氟沙星耐药性的调节作用

Modulation of the norfloxacin resistance in Staphylococcus aureus by Croton campestris A. and Ocimum gratissimum L.

作者信息

Coutinho Henrique D M, Matias Edinardo F F, Santos Karla K A, Santos Francisco A V, Morais-Braga Maria Flaviana B, Souza Teógenes M, Andrade Jacqueline Cosmo, Souza Celestina E S, Tintino Saulo R, Guedes Gláucia M M, Falcão-Silva Vivyanne S, Siqueira-Júnior José P, Costa José G M

机构信息

Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato, CE, Brasil.

出版信息

Biomedica. 2011 Oct-Dec;31(4):608-12. doi: 10.1590/S0120-41572011000400016.

DOI:10.1590/S0120-41572011000400016

PMID:22674372

Abstract

INTRODUCTION

Some species of Staphylococcus are often recognized as etiological agents of many animal and human opportunistic infections. This study is the first test of change in resistance of antibiotic activity by Croton campestris A. and Ocimum gratissimum L. against multiresistant strains of Staphylococcus aureus.

OBJECTIVE

In this study, the hexane and methanol extract of Croton campestris A. and Ocimum gratissimum L. was tested for antibacterial activity alone and in combination with norfloxacin against the strain SA1199B.

MATERIALS AND METHODS

The minimum inhibitory concentration (MIC) and the modulatory effect of extracts was assayed using microtitre assay.

RESULTS

By the fact of the MIC observed was not clinically relevant (MIC= 512 to ≥1.024 µg/ml), the antibiotic activity of norfloxacin was enhanced when this antibiotic was combined with sub-inhibitory concentrations of extracts, mainly the hexane extracts.

CONCLUSIONS

These results indicate that the assayed extracts present compounds that can be used as a putative efflux pump inhibitor, indicating that Croton campestris A. and Ocimum gratissimum L. can be a source of plant derived products with antibiotic modifier activity.

摘要