用黄槐决明调控金黄色葡萄球菌的诺氟沙星耐药性
Modulation of the norfloxacin resistance in Staphylococcus aureus by Cordia verbenaceae DC.
机构信息
Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato, Ceara, Brazil.
出版信息
Indian J Med Res. 2013 Jan;137(1):178-82.
BACKGROUND & OBJECTIVES: Several chemical compounds isolated from natural sources have antibacterial activity and some enhance the antibacterial activity of antibiotics reversing the natural resistance of bacteria to certain antibiotics. In this study, the hexane and methanol extract of Cordia verbenaceae were assessed for antibacterial activity alone and combinated with norfloxacin against the Staphylococcus aureus strain SA1199B.
METHODS
The minimum inhibitory concentration (MIC) of extracts was assayed using microdilution assay and the modulatory activity was evaluated using plate diffusion assay.
RESULTS
The MIC observed varied between 256 to >1024 μg/ml. However, the antibiotic activity of norfloxacin was enhanced in the presence of subinhibitory concentrations of hexane extract of C. verbenaceae (HECV). INTERPRETATIONS & CONCLUSIONS: Our results indicate that Cordia verbenaceae DC. can be a source of plant derived products with antibiotic modifying activity.
背景与目的
从天然来源中分离出的几种化学化合物具有抗菌活性，有些化合物能增强抗生素的抗菌活性，逆转细菌对某些抗生素的天然耐药性。在这项研究中，评估了马鞭草科的心叶 Cordia verbenaceae 的正己烷和甲醇提取物单独使用以及与诺氟沙星联合使用时对金黄色葡萄球菌 SA1199B 菌株的抗菌活性。
方法
采用微量稀释法测定提取物的最小抑菌浓度（MIC），采用平板扩散法评价调节活性。
结果
观察到的 MIC 值在 256 至>1024 μg/ml 之间变化。然而，在亚抑制浓度的心叶 Cordia verbenaceae DC.（HECV）正己烷提取物存在下，诺氟沙星的抗生素活性增强。
解释与结论
我们的结果表明，马鞭草科的心叶 Cordia verbenaceae DC. 可能是具有抗生素修饰活性的植物衍生产品的来源。
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