Research Institute for Biological Functions, Chubu University, Kasugai, Aichi, Japan.
Biofactors. 2012 Jul-Aug;38(4):300-8. doi: 10.1002/biof.1022. Epub 2012 Jun 2.
This study examined the effects of fargesin, a neolignan isolated from Magnolia plants, on obesity and insulin resistance and the possible mechanisms involved in these effects in 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. Fargesin promoted the glucose uptake in 3T3-L1 adipocytes. In HFD-induced obese mice, fargesin decreased the body weight gain, white adipose tissue (WAT), and plasma triglyceride, non-esterified fatty acid and glucose levels, and improved the glucose tolerance. Fargesin increased glucose transporter 4 (GLUT4) protein expression and phosphorylation of Akt, AMP-activated protein kinase (AMPK), and acetyl-CoA carboxylase (ACC) in both 3T3-L1 adipocytes and WAT of HFD-induced obese mice. Fargesin also decreased the mRNA expression levels of fatty acid oxidation-related genes, such as peroxisome proliferator-activated receptor α (PPARα), carnitine palmitoyltransferase-1 (CPT-1), uncoupling protein-2 (UCP-2) and leptin in WAT. Taken together, the present findings suggest that fargesin improves dyslipidemia and hyperglycemia by activating Akt and AMPK in WAT.
本研究探讨了从木兰属植物中分离得到的新木脂素法呢醇对肥胖和胰岛素抵抗的影响，以及其在 3T3-L1 脂肪细胞和高脂饮食（HFD）诱导肥胖小鼠中产生这些作用的可能机制。法呢醇可促进 3T3-L1 脂肪细胞的葡萄糖摄取。在 HFD 诱导的肥胖小鼠中，法呢醇可降低体重增加、白色脂肪组织（WAT）和血浆甘油三酯、非酯化脂肪酸和葡萄糖水平，并改善葡萄糖耐量。法呢醇可增加 3T3-L1 脂肪细胞和 HFD 诱导肥胖小鼠 WAT 中的葡萄糖转运蛋白 4（GLUT4）蛋白表达以及 Akt、AMP 激活的蛋白激酶（AMPK）和乙酰辅酶 A 羧化酶（ACC）的磷酸化。法呢醇还可降低 WAT 中脂肪酸氧化相关基因的 mRNA 表达水平，如过氧化物酶体增殖物激活受体 α（PPARα）、肉碱棕榈酰转移酶-1（CPT-1）、解偶联蛋白-2（UCP-2）和瘦素。综上所述，本研究结果表明，法呢醇通过激活 WAT 中的 Akt 和 AMPK 来改善血脂异常和高血糖。
