细胞色素P450的多态性、表达水平以及分离株对伊曲康唑的敏感性。
Polymorphism and expression level of (cytochrome P450) and sensitivity to ipconazole in isolates.
作者信息
Tateishi Hideaki, Miyake Taiji, Suga Haruhisa
机构信息
Organic Synthesis Research Laboratories, Agrochemicals Department, Kureha Corporation, 16 Ochiai, Nishiki-machi, Iwaki, Fukushima, 974-8686, Japan.
Agrochemicals Department, Kureha Corporation, 3-26-2 Hyakunin-cho, Shinjuku, Tokyo 169-8503, Japan.
出版信息
J Pestic Sci. 2019 Feb 20;44(1):25-32. doi: 10.1584/jpestics.D18-011.
The relationship between the nucleotide sequences of , its expression level and its sensitivity to ipconazole of isolates were investigated. Single nucleotide polymorphisms (SNPs) were observed in the of isolates with different sensitivities to ipconazole, but no amino acid substitution was detected in the putative amino acid sequence of the CYP51 protein. On the other hand, the expression of was enhanced by the presence of ipconazole, and it tended to be higher in isolates with lower sensitivities and no gibberellin productivity. In the presumed promoter region, the upstream nucleotide sequence of , several common SNPs and insertions of nucleotides were detected in the lower sensitivity isolates. These results suggest that isolates consist of 2 different groups in sensitivity and gibberellin productivity, and no amino acid substitution in CYP51 protein may contribute to the stably high efficacy of ipconazole against rice Bakanae disease for more than 25 years.
研究了稻瘟病菌分离株的核苷酸序列、其表达水平与其对抑霉唑敏感性之间的关系。在对抑霉唑敏感性不同的分离株的 中观察到单核苷酸多态性（SNP），但在 CYP51 蛋白的推定氨基酸序列中未检测到氨基酸取代。另一方面，抑霉唑的存在增强了 的表达，并且在敏感性较低且无赤霉素产生能力的分离株中其表达往往更高。在假定的启动子区域，即 的上游核苷酸序列中，在敏感性较低的分离株中检测到几个常见的 SNP 和核苷酸插入。这些结果表明，稻瘟病菌分离株在敏感性和赤霉素产生能力方面由 2 个不同的组组成，并且 CYP51 蛋白中无氨基酸取代可能有助于抑霉唑在超过 25 年的时间里对水稻恶苗病保持稳定的高防治效果。
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