Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Insect Mol Biol. 2012 Aug;21(4):422-36. doi: 10.1111/j.1365-2583.2012.01148.x. Epub 2012 Jun 8.
The citrus red mite, Panonychus citri, is known for its ability rapidly to evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. In this study, we constructed an unprecedented four gigabase pair transcriptome of P. citri, which was assembled into 64 149 unique transcripts, the functions of which were annotated by five public databases. A total of 116 unique transcripts were identified as representatives of potential involvement in the detoxification of xenobiotics. Genes recorded to encoding insecticide/acaricide target proteins were also obtained from the P. citri transcriptome. In order to explore novel candidate genes potentially involved in the pesticide detoxification of P. citri, we also constructed digital gene expression libraries of short-term transcriptome responses of P. citri to pesticides, which resulted in the identification of 120 unique transcripts potentially associated with insecticide/acaricide detoxification. Our study will facilitate molecular research on pesticide resistance in citrus red mites, as well as in other phytophagous mites.
柑橘全爪螨（Panonychus citri）以其快速产生抗药性和适应产生毒素的宿主的能力而闻名。在这项研究中，我们构建了一个空前的柑橘全爪螨四千兆碱基对转录组，它被组装成 64149 个独特的转录本，这些转录本的功能由五个公共数据库进行注释。共有 116 个独特的转录本被鉴定为潜在参与外来化合物解毒的代表。从柑橘全爪螨转录组中还获得了记录编码杀虫剂/杀螨剂靶蛋白的基因。为了探索可能参与柑橘全爪螨农药解毒的新候选基因，我们还构建了柑橘全爪螨短期转录组对农药反应的数字基因表达文库，鉴定出 120 个可能与杀虫剂/杀螨剂解毒相关的独特转录本。我们的研究将促进柑橘全爪螨以及其他植食性螨类的农药抗性的分子研究。
