Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, P. R. China.
Arch Insect Biochem Physiol. 2013 Apr;82(4):213-26. doi: 10.1002/arch.21087. Epub 2013 Feb 12.
The citrus red mite, Panonychus citri, is known for its ability to rapidly evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. To get better insight into the detoxification mechanism of P. citri, two carboxylesterase (CarE) genes, PCE1 and PCE2, were isolated and characterized. PCE1 and PCE2 contained open reading frames of 1,653 and 1,392 nucleotides, encoding proteins of 550 and 463 amino acid residues, respectively. Phylogenetic analyses showed that PCE1 and PCE2 were most closely related to the CarE genes from other phytophagous mites. The transcriptional profiles of two CarE genes among developmental stages (egg, larva, nymph, adult female, and adult male), after exposing to four acaricides (avermectin, azocyclotin, pyridaben, and spirodiclofen) and acid rain were investigated using real-time quantitative PCR (qPCR). The results showed that during development, PCE1 was highly expressed at the egg stage, whereas PCE2 was abundantly expressed at the adult stage of males. The expression levels of PCE1 were highly induced upon exposure to acaricides and acid rain. On the other hand, the expression levels of PCE2 were increased after treatment with avermectin and pyridaben. These results suggest that PCE1 and PCE2 may have distinct roles in different developmental stages and participate in the detoxification of acaricides.
柑橘全爪螨（Panonychus citri）以其快速进化出对杀虫剂/杀螨剂的抗性以及适应产生毒素的宿主的能力而闻名。为了更好地了解柑橘全爪螨的解毒机制，分离并鉴定了两个羧酸酯酶（CarE）基因，PCE1 和 PCE2。PCE1 和 PCE2 分别包含 1653 个和 1392 个核苷酸的开放阅读框，分别编码 550 个和 463 个氨基酸残基的蛋白质。系统发育分析表明，PCE1 和 PCE2 与其他植食性螨的 CarE 基因最为密切相关。使用实时定量 PCR（qPCR）研究了两个 CarE 基因在发育阶段（卵、幼虫、若虫、雌成虫和雄成虫）以及暴露于四种杀螨剂（阿维菌素、唑虫酰胺、哒螨灵和螺虫乙酯）和酸雨后的转录谱。结果表明，在发育过程中，PCE1 在卵期高度表达，而 PCE2 在雄性成虫期大量表达。PCE1 的表达水平在暴露于杀螨剂和酸雨后高度诱导。另一方面，PCE2 的表达水平在使用阿维菌素和哒螨灵处理后增加。这些结果表明，PCE1 和 PCE2 可能在不同的发育阶段发挥不同的作用，并参与杀螨剂的解毒。
