Ereqat Suheir, Nasereddin Abedelmajeed, Azmi Kifaya, Abdeen Ziad, Greenblatt Charles L, Spigelman Mark, Rastogi Nalin, Bar-Gal Gila Kahila
Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, Palestine.
BMC Res Notes. 2012 Jun 7;5:270. doi: 10.1186/1756-0500-5-270.
The World Health Organization (WHO) declared human tuberculosis (TB) a global health emergency and launched the "Global Plan to Stop Tuberculosis" which aims to save a million lives by 2015. Global control of TB is increasingly dependent on rapid and accurate genetic typing of species of the Mycobacterium tuberculosis (MTB) complex including M. tuberculosis. The aim of this study was to identify and genetically characterize the MTB isolates circulating in the West Bank, Palestinian Territories. Genotyping of the MTB isolates from patients with pulmonary TB was carried out using two molecular genetic techniques, spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) supported by analysis of the MTB specific deletion 1 (TbD1).
A total of 17 MTB patterns were obtained from the 31 clinical isolates analyzed by spoligotyping; corresponding to 2 orphans and 15 shared-types (SITs). Fourteen SITs matched a preexisting shared-type in the SITVIT2 database, whereas a single shared-type SIT3348 was newly created. The most common spoligotyping profile was SIT53 (T1 variant), identified in 35.5 % of the TB cases studied. Genetic characterization of 22 clinical isolates via the 15 loci MIRU-VNTR typing distinguished 19 patterns. The 15-loci MIT144 and MIT145 were newly created within this study. Both methods determined the present of M. bovis strains among the isolates.
Significant diversity among the MTB isolates circulating in the West Bank was identified with SIT53-T1 genotype being the most frequent strain. Our results are used as reference database of the strains circulating in our region and may facilitate the implementation of an efficient TB control program.
世界卫生组织（WHO）宣布人类结核病（TB）为全球卫生紧急情况，并发起了“全球防治结核病计划”，目标是到2015年挽救100万人的生命。全球结核病控制越来越依赖于对结核分枝杆菌（MTB）复合群（包括结核分枝杆菌）进行快速准确的基因分型。本研究的目的是鉴定巴勒斯坦领土西岸流行的MTB分离株并对其进行基因特征分析。采用两种分子遗传学技术，即间隔寡核苷酸分型（spoligotyping）和分枝杆菌插入重复单位-可变数目串联重复序列（MIRU-VNTR），并结合MTB特异性缺失1（TbD1）分析，对肺结核患者的MTB分离株进行基因分型。
通过间隔寡核苷酸分型对31株临床分离株进行分析，共获得17种MTB模式；对应于2个孤儿型和15个共享型（SITs）。14个SITs与SITVIT2数据库中已有的共享型匹配，而一个共享型SIT3348是新创建的。最常见的间隔寡核苷酸分型图谱是SIT53（T1变异型），在所研究的结核病病例中占35.5%。通过15个位点的MIRU-VNTR分型对22株临床分离株进行基因特征分析，区分出19种模式。在本研究中，15个位点的MIT144和MIT145是新创建的。两种方法都确定了分离株中存在牛分枝杆菌菌株。
确定了西岸流行的MTB分离株具有显著多样性，SIT53-T1基因型是最常见的菌株。我们的结果用作我们地区流行菌株的参考数据库，可能有助于实施有效的结核病控制计划。
