维生素 D 状况与肥胖儿童的氧化应激、炎症和内皮细胞激活的生物标志物有关。

Vitamin D status is linked to biomarkers of oxidative stress, inflammation, and endothelial activation in obese children.

机构信息

Department of Pediatrics, Dr Peset University Hospital, Valencia, Spain.

出版信息

J Pediatr. 2012 Nov;161(5):848-54. doi: 10.1016/j.jpeds.2012.04.046. Epub 2012 Jun 5.

DOI:10.1016/j.jpeds.2012.04.046

PMID:22677566

Abstract

OBJECTIVE

To examine vitamin D, parathyroid hormone, and serum calcium-phosphorus levels relationships to biomarkers of oxidative/nitrosative stress, inflammation, and endothelial activation, potential contributors for vascular complications in obese children.

STUDY DESIGN

Cross-sectional clinical study of 66 obese Caucasian children aged 7 to 14 years. Cardiovascular risk factors were assessed. Malondialdehyde and myeloperoxidase as measures of oxidative stress, and plasma nitrite+nitrate, urinary nitrate, and 3-nitrotyrosine as markers of nitrosative stress were measured. Adipocytokines, inflammatory molecules (high-sensitivity C-reactive protein, interleukin-6, and tumor necrosis factor-α), endothelial activation molecules (soluble intercellular adhesion molecule-1, soluble vascular cell adhesion molecule 1 [sVCAM-1]), E-selectin, and vascular endothelial growth factor were also investigated. Serum 25-hydroxy-cholecalciferol [25(OH)D], intact parathormone, and calcium-phosphorus levels were determined in these children and in a comparison group of 39 non-obese children.

RESULTS

Obese children had a significantly lower 25(OH)D level (P = .002) and a higher intact parathormone (P = .011) than non-obese children. Phosphorus and the calcium-phosphorus product were also significantly higher (P < .0001). Insufficient serum concentrations of 25(OH)D (<20 ng/mL) were detected in 5% of normal children and in 30% of the obese children. In the obese children with vitamin D insufficiency, malondialdehyde, myeloperoxidase, 3-nitrotyrosine, interleukin-6, and sVCAM-1 were substantially elevated. A partial correlation analysis showed an inverse relationship of 25(OH)D levels with 3-nitrotyrosine (r = -0.424, P = .001), and sVCAM-1 (r = -0.272, P = .032).

CONCLUSIONS

Insufficient 25(OH)D levels were detected in severely obese children with increased markers of oxidative/nitrosative stress, inflammation, and endothelial activation.

摘要

目的

研究维生素 D、甲状旁腺激素和血清钙磷水平与氧化/硝化应激、炎症和内皮细胞激活生物标志物的关系，这些生物标志物可能是肥胖儿童血管并发症的潜在因素。

研究设计

对 66 名 7 至 14 岁的白种肥胖儿童进行横断面临床研究。评估心血管危险因素。测量丙二醛和髓过氧化物酶作为氧化应激的指标，以及血浆硝酸盐+亚硝酸盐、尿硝酸盐和 3-硝基酪氨酸作为硝化应激的标志物。还研究了脂肪细胞因子、炎症分子（高敏 C 反应蛋白、白细胞介素-6 和肿瘤坏死因子-α）、内皮细胞激活分子（可溶性细胞间黏附分子-1、可溶性血管细胞黏附分子 1[sVCAM-1]）、E-选择素和血管内皮生长因子。这些儿童和 39 名非肥胖儿童的血清 25-羟胆钙化醇[25（OH）D]、完整甲状旁腺激素和钙磷水平也进行了测定。

结果

与非肥胖儿童相比，肥胖儿童的 25（OH）D 水平显著降低（P =.002），完整甲状旁腺激素水平显著升高（P =.011）。磷和钙磷乘积也显著升高（P <.0001）。正常儿童中 5%、肥胖儿童中 30%存在血清 25（OH）D 浓度不足（<20ng/mL）。在维生素 D 不足的肥胖儿童中，丙二醛、髓过氧化物酶、3-硝基酪氨酸、白细胞介素-6 和 sVCAM-1 显著升高。偏相关分析显示 25（OH）D 水平与 3-硝基酪氨酸（r = -0.424，P =.001）和 sVCAM-1（r = -0.272，P =.032）呈负相关。