Department of Oral and Maxillofacial Pathology, SGT Dental College and Hospital, Haryana, India.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Feb;113(2):245-53. doi: 10.1016/j.tripleo.2011.05.034. Epub 2011 Aug 27.
Oral squamous cell carcinoma (OSCC) may be preceded by potentially malignant disorders such as leukoplakia and oral submucous fibrosis (OSF) and has a greater than normal risk of malignant transformation. Very little is known of cytokeratin (CK) alterations in OSF, leukoplakia, and OSCC. The aim of this study was to evaluate and compare the expression of CK8 and CK18 in normal oral mucosa, oral epithelial dysplasia, OSF, and OSCC by immunohistochemistry.
Paraffin-embedded normal (n = 10), dysplasia (n = 10), OSF (n = 10), and OSCC (n = 10) tissues were stained with CK8 and CK18 by immunohistochemistry.
Increased expression of CK8 and CK18 was seen in dysplasia, OSF, and OSCC. Staining pattern and intensity showed variations, with intensity of staining in basal and suprabasal layers for CK8 and CK18.
Intensity of staining in the basal layer for CK18 was statistical significant, suggesting CK8 and CK18 as surrogate markers of malignant transformation.
口腔鳞状细胞癌（OSCC）可能以前存在癌前病变，如白斑病和口腔黏膜下纤维性变（OSF），并且恶性转化的风险高于正常。关于 OSF、白斑病和 OSCC 中细胞角蛋白（CK）改变的知识非常有限。本研究旨在通过免疫组织化学评估和比较正常口腔黏膜、口腔上皮异型增生、OSF 和 OSCC 中 CK8 和 CK18 的表达。
用免疫组织化学法对石蜡包埋的正常（n = 10）、异型增生（n = 10）、OSF（n = 10）和 OSCC（n = 10）组织进行 CK8 和 CK18 染色。
在异型增生、OSF 和 OSCC 中观察到 CK8 和 CK18 的表达增加。染色模式和强度存在差异，CK8 和 CK18 在基底和超基底层的染色强度。
CK18 在基底层的染色强度具有统计学意义，提示 CK8 和 CK18 可作为恶性转化的替代标志物。
