Department of Dermatology and Venereology, Peking University First Hospital, No. 8, Xi-Shi-Ku St., Xicheng District, Beijing 100034, People's Republic of China.
Mycopathologia. 2012 Oct;174(4):273-82. doi: 10.1007/s11046-012-9557-4. Epub 2012 Jun 8.
Aspergillus fumigatus is naturally exposed to a highly variable environment and subjected to various kinds of stresses. High-osmolarity glycerol mitogen-activated protein kinase (HOG-MAPK) pathway plays a crucial role in regulating cellular homeostasis in response to environmental changes. Here, we explored the contribution of HOG-MAPK pathway to the adaptive responses to thermal stress and other related stresses in A. fumigatus. We observed the phenotype features of wild-type strains and their derived mutants at 37 and 48 °C, and the results suggested that tcsB participates in response to high temperature. Furthermore, susceptibility test for antifungal drugs showed that SHO1 branch is probably involved in the susceptibility of A. fumigatus to itraconazole at high temperature. Although sakA expression at mRNA level appeared unchanged in wild-type AF293 subjected to thermal stress, phosphorylated SakAp level increased significantly in the strains exposed to cold stress, 250 mmol/L nystatin or 10 % dimethyl sulfoxide in a manner dependent on the SLN1 branch and independent on the SHO1 branch. Taken together, these results indicate that HOG-MAPK pathway, especially the SLN1 branch, plays an important role in the adaptations of A. fumigatus to thermal stress and other related stresses.
烟曲霉自然暴露于高度可变的环境中，并受到各种压力的影响。高渗透压甘油有丝分裂原激活蛋白激酶（HOG-MAPK）途径在调节细胞内稳态以响应环境变化方面起着至关重要的作用。在这里，我们探讨了 HOG-MAPK 途径对烟曲霉适应热应激和其他相关应激的贡献。我们观察了野生型菌株及其衍生突变体在 37 和 48°C 下的表型特征，结果表明 tcsB 参与了对高温的反应。此外，抗真菌药物敏感性测试表明，SHO1 分支可能参与了烟曲霉对高温下伊曲康唑的敏感性。尽管在受到热应激的野生型 AF293 中，sakA 在 mRNA 水平上的表达似乎没有改变，但在受到冷应激、250mmol/L 制霉菌素或 10%二甲基亚砜暴露的菌株中，磷酸化 SakAp 水平显著增加，这种增加方式依赖于 SLN1 分支，而不依赖于 SHO1 分支。总之，这些结果表明 HOG-MAPK 途径，特别是 SLN1 分支，在烟曲霉适应热应激和其他相关应激方面起着重要作用。
