Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang, 330013, China.
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu, 610064, China.
World J Microbiol Biotechnol. 2018 Feb 9;34(2):35. doi: 10.1007/s11274-018-2419-6.
The High osmolarity glycerol (HOG) gene family plays crucial roles in various developmental and physiological processes in fungi, such as the permeability of cell membrane, chlamydospore formation and stress signaling. Although the function of HOG genes has been investigated in Saccharomyces cerevisiae and some filamentous fungi, a comprehensive analysis of HOG gene family has not been performed in Aspergillus oryzae, a fungi mainly used for the production of soy sauce. In this study, we identified and corrected a total of 90 HOG genes from the A. oryzae genome. According to the phylogenetic relationship, they were divided into four discrete groups (Group A-D) comprising of 16, 24, 30 and 20 proteins, respectively. Six conserved motifs and exon-intron structures were examined among all HOG proteins to reveal the diversity of AoHOG genes. Based on transcriptome technology, the expression patterns of AoHOG genes across all developmental stages was identified, suggesting that the AoHOG gene family mainly functions in the logarithmic phase of development. The expression profiles of AoHOG genes under different concentrations of salt stress indicated that AoHOG genes are extensively involved in salt stress response, with possibly different mechanisms. The genome-wide identification, evolutionary, gene structures and expression analyses of AoHOG genes provide a comprehensive overview of this gene family as well as their potential involvements in development and stress responses. Our results will facilitate further research on HOG gene family regarding their physiological and biochemical functions.
高渗透压甘油（HOG）基因家族在真菌的各种发育和生理过程中发挥着关键作用，例如细胞膜的通透性、厚垣孢子的形成和应激信号转导。虽然 HOG 基因的功能已在酿酒酵母和一些丝状真菌中进行了研究，但在酱油生产中主要使用的真菌米曲霉中，尚未对 HOG 基因家族进行全面分析。在本研究中，我们从米曲霉基因组中鉴定并纠正了总共 90 个 HOG 基因。根据系统发育关系，它们分为四个离散的组（A-D 组），分别包含 16、24、30 和 20 个蛋白。在所有 HOG 蛋白中检查了六个保守基序和外显子-内含子结构，以揭示 AoHOG 基因的多样性。基于转录组技术，鉴定了 AoHOG 基因在所有发育阶段的表达模式，表明 AoHOG 基因家族主要在对数生长期发挥作用。AoHOG 基因在不同盐浓度胁迫下的表达谱表明，AoHOG 基因广泛参与盐胁迫反应，可能具有不同的机制。AoHOG 基因的全基因组鉴定、进化、基因结构和表达分析提供了对该基因家族的全面概述，以及它们在发育和应激反应中的潜在参与。我们的研究结果将有助于进一步研究 HOG 基因家族的生理生化功能。
