Højland Torben, Veedu Rakesh N, Vester Birte, Wengel Jesper
Department of Physics, Chemistry and Pharmacy, Nucleic Acid Center, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Artif DNA PNA XNA. 2012 Jan-Mar;3(1):14-21. doi: 10.4161/adna.19272.
We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5'-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°N(m), Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by all four polymerases, but they were unable to perform consecutive incorporations of α-L-LNA nucleotides. In addition it was found that primer extension can be achieved using templates containing one α-L-LNA nucleotide.
我们描述了首次将α-L-锁核酸核苷酸酶促掺入寡核苷酸的过程。发现α-L-锁核酸的5'-三磷酸是DNA聚合酶KOD、9°N(m)、Phusion和HIV逆转录酶的底物。所有这四种聚合酶均可将三个分散的α-L-锁核酸胸腺嘧啶核苷酸掺入DNA链中，但它们无法连续掺入α-L-锁核酸核苷酸。此外，还发现使用含有一个α-L-锁核酸核苷酸的模板可实现引物延伸。
