使用锁核酸三磷酸核苷酸的聚合酶链反应和转录
Polymerase chain reaction and transcription using locked nucleic acid nucleotide triphosphates.
作者信息
Veedu Rakesh N, Vester Birte, Wengel Jesper
机构信息
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, 5230, Denmark.
出版信息
J Am Chem Soc. 2008 Jul 2;130(26):8124-5. doi: 10.1021/ja801389n. Epub 2008 Jun 6.
PMID:18533656
Abstract
Polymerase chain reaction amplification of a locked nucleic acid (LNA)-modified DNA strand and transcription reactions using LNA-A nucleoside 5'-triphosphate were successfully accomplished with DNA and RNA polymerases.
摘要
使用DNA和RNA聚合酶成功完成了对锁核酸（LNA）修饰的DNA链的聚合酶链反应扩增以及使用LNA-A核苷5'-三磷酸的转录反应。
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