Netsvetaev V P, Akinshina O V, Bondarenko L S
Genetika. 2012 Mar;48(3):401-4.
alpha-Amylase isozymes were detected via electrophoretic separation in a Tris-glycine polyacrylamide gel system (pH 8.4). Three chromosome 6B loci controlling the alpha-amylase isozyme composition were identified by studying the grain alpha-amylase patterns in an F --> infinity self-pollinating population of winter common wheat (Donskoi Mayak). The loci were found to take the following order in the long arm of chromosome 6B: cen.-alpha-Amy-B3-alpha-Amy-B6-alpha-Amy-B1-.
通过在Tris-甘氨酸聚丙烯酰胺凝胶系统(pH 8.4)中进行电泳分离来检测α-淀粉酶同工酶。通过研究冬小麦(顿斯科伊·玛雅克)F→∞自花授粉群体中的籽粒α-淀粉酶模式,鉴定出了三个控制α-淀粉酶同工酶组成的6B染色体位点。发现这些位点在6B染色体长臂上的排列顺序如下:着丝粒-α-Amy-B3-α-Amy-B6-α-Amy-B1-。