Department of Mathematics, Imperial College London, London, UK.
Trends Microbiol. 2012 Aug;20(8):376-84. doi: 10.1016/j.tim.2012.05.001. Epub 2012 Jun 7.
Cells live in an ever-changing environment and continuously sense, process and react to environmental signals using their inherent signaling and gene regulatory networks. Recently, there have been great advances on rewiring the native cell signaling and gene networks to program cells to sense multiple noncognate signals and integrate them in a logical manner before initiating a desired response. Here, we summarize the current state-of-the-art of engineering synthetic genetic logic circuits to customize cellular signaling behaviors, and discuss their promising applications in biocomputing, environmental, biotechnological and biomedical areas as well as the remaining challenges in this growing field.
细胞生活在不断变化的环境中，使用其内在的信号转导和基因调控网络持续感知、处理和响应环境信号。最近，人们在重新布线固有细胞信号和基因网络方面取得了重大进展，能够对细胞进行编程，使其能够感知多种非同源信号，并以逻辑方式对其进行整合，然后再启动所需的响应。在这里，我们总结了工程合成遗传逻辑电路以定制细胞信号转导行为的最新进展，并讨论了它们在生物计算、环境、生物技术和生物医学领域的广阔应用前景，以及这一日益发展的领域中存在的剩余挑战。
