Department of Genetics, Cellular Biology, and Development, University of Minnesota, Twin Cities, Minneapolis, MN, USA.
Department of Computer Science, University of Minnesota, Twin Cities, Minneapolis, MN, USA.
Nat Commun. 2023 Apr 20;14(1):2257. doi: 10.1038/s41467-023-37752-x.
Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here we present a biocomputing technology called Trumpet: Transcriptional RNA Universal Multi-Purpose GatE PlaTform. Trumpet combines the simplicity and robustness of the simplest in vitro biocomputing methods, adding signal amplification and programmability, while avoiding common shortcomings of live cell-based biocomputing solutions. We have demonstrated the use of Trumpet to build all universal Boolean logic gates. We have also built a web-based platform for designing Trumpet gates and created a primitive processor by networking several gates as a proof-of-principle for future development. The Trumpet offers a change of paradigm in biocomputing, providing an efficient and easily programmable biological logic gate operating system.
生物计算正在成为一种可行且快速发展的传统电子计算替代方案。在这里，我们提出了一种名为 Trumpet 的生物计算技术：转录 RNA 通用多用途 GatE PlaTform。Trumpet 结合了最简单的体外生物计算方法的简单性和鲁棒性，同时添加了信号放大和可编程性，避免了基于活细胞的生物计算解决方案的常见缺点。我们已经证明了使用 Trumpet 来构建所有通用布尔逻辑门。我们还构建了一个基于网络的 Trumpet 门设计平台，并通过将多个门联网创建了一个原始处理器，作为未来开发的原理证明。Trumpet 为生物计算提供了一种范式转变，提供了一种高效且易于编程的生物逻辑门操作系统。
