Suppr超能文献

通过一种新的比色测定法检测硫代次黄苷酸和硫唑嘌呤对5-氨基咪唑-4-甲酰胺核苷酸转甲酰酶的抑制作用。

Detection of inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase by thioinosinic acid and azathioprine by a new colorimetric assay.

作者信息

Ha T, Morgan S L, Vaughn W H, Eto I, Baggott J E

机构信息

Department of Nutrition Sciences, University of Alabama, Birmingham 35294.

出版信息

Biochem J. 1990 Dec 1;272(2):339-42. doi: 10.1042/bj2720339.

DOI:10.1042/bj2720339
PMID:2268263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149705/
Abstract

The colorimetric assay for 5-aminoimidazole-4-carboxamide ribotide (AICAR) transformylase (phosphoribosylamino-imidazolecarboxamide formyltransferase; EC 2.1.2.3) has been extensively modified. The modified assay is based upon the short-term permanganate oxidation of the folate product, tetrahydrofolate (H4folate) to p-aminobenzoyl glutamate (pABG). The modified assay was used to detect the transformylase activity in crude extracts of peripheral-blood mononuclear cells (PBMCs). Azathioprine and its metabolite, thioinosinic acid (tIMP), are competitive inhibitors (with respect to AICAR) of the chicken liver transformylase and the transformylase from PBMCs of the MRL/lpr mouse, an animal model of systemic autoimmune disease. The Ki values of tIMP and azathioprine for the chicken liver enzyme are 39 +/- 4 microM and 120 +/- 10 microM, whereas the Ki values for the enzyme from PBMCs of the MRL/lpr mouse are 110 +/- 20 microM and 90 +/- 14 microM respectively. The anti-inflammatory drugs ibuprofen and naproxen are also inhibitors of the transformylase.

摘要

用于5-氨基咪唑-4-甲酰胺核苷酸（AICAR）转甲酰基酶（磷酸核糖氨基咪唑甲酰胺甲酰基转移酶；EC 2.1.2.3）的比色测定法已得到广泛改进。改进后的测定法基于叶酸产物四氢叶酸（H4叶酸）短期被高锰酸盐氧化为对氨基苯甲酰谷氨酸（pABG）。改进后的测定法用于检测外周血单个核细胞（PBMC）粗提物中的转甲酰基酶活性。硫唑嘌呤及其代谢产物硫代次黄嘌呤核苷酸（tIMP）是鸡肝转甲酰基酶和MRL/lpr小鼠（一种系统性自身免疫疾病动物模型）PBMC中转甲酰基酶（相对于AICAR）的竞争性抑制剂。tIMP和硫唑嘌呤对鸡肝酶的Ki值分别为39±4微摩尔和120±10微摩尔，而对MRL/lpr小鼠PBMC中酶的Ki值分别为110±20微摩尔和90±14微摩尔。抗炎药布洛芬和萘普生也是转甲酰基酶的抑制剂。

相似文献

1
Detection of inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase by thioinosinic acid and azathioprine by a new colorimetric assay.通过一种新的比色测定法检测硫代次黄苷酸和硫唑嘌呤对5-氨基咪唑-4-甲酰胺核苷酸转甲酰酶的抑制作用。
Biochem J. 1990 Dec 1;272(2):339-42. doi: 10.1042/bj2720339.
2
Inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase, adenosine deaminase and 5'-adenylate deaminase by polyglutamates of methotrexate and oxidized folates and by 5-aminoimidazole-4-carboxamide riboside and ribotide.甲氨蝶呤和氧化叶酸的聚谷氨酸酯以及5-氨基咪唑-4-甲酰胺核苷和核苷酸对5-氨基咪唑-4-甲酰胺核糖核苷酸转甲酰酶、腺苷脱氨酶和5'-腺苷酸脱氨酶的抑制作用
Biochem J. 1986 May 15;236(1):193-200. doi: 10.1042/bj2360193.
3
Inhibition of chicken liver 5-aminoimidazole-4-carboxamide ribonucleotide transformylase by 5,8-dideaza analogues of folic acid.叶酸的5,8-二脱氮类似物对鸡肝5-氨基咪唑-4-甲酰胺核糖核苷酸转甲酰酶的抑制作用。
Biochem Pharmacol. 1988 Feb 1;37(3):449-51. doi: 10.1016/0006-2952(88)90213-4.
4
Radioassay of bifunctional 5-aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase by thin-layer chromatography.通过薄层色谱法对双功能5-氨基咪唑-4-甲酰胺核苷酸转甲酰基酶-IMP环水解酶进行放射测定。
Anal Biochem. 1994 Sep;221(2):401-4. doi: 10.1006/abio.1994.1433.
5
On the purification and mechanism of action of 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase from chicken liver.鸡肝中5-氨基咪唑-4-甲酰胺-核糖核苷酸转甲酰酶的纯化及作用机制
Biochemistry. 1981 Jan 20;20(2):337-44. doi: 10.1021/bi00505a017.
6
On the cofactor specificity of glycinamide ribonucleotide and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase from chicken liver.关于鸡肝中甘氨酰胺核糖核苷酸和5-氨基咪唑-4-甲酰胺核糖核苷酸转甲酰酶的辅因子特异性
Biochemistry. 1981 Mar 3;20(5):1241-5. doi: 10.1021/bi00508a029.
7
5-Aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase from human CCRF-CEM leukemia cells: purification, pH dependence, and inhibitors.来自人CCRF-CEM白血病细胞的5-氨基咪唑-4-甲酰胺核苷酸转甲酰酶-IMP环水解酶：纯化、pH依赖性及抑制剂
Biochemistry. 1994 Nov 29;33(47):14237-45. doi: 10.1021/bi00251a036.
8
Cofactor role for 10-formyldihydrofolic acid.10-甲酰基二氢叶酸的辅助因子作用。
Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):1031-6. doi: 10.1042/bj3081031.
9
Antifolates induce primary inhibition of the de novo purine pathway prior to 5-aminoimidazole-4-carboxamide ribotide transformylase in leukemia cells.抗叶酸药物在白血病细胞中，于5-氨基咪唑-4-甲酰胺核苷酸转甲酰酶之前对嘌呤从头合成途径产生初级抑制作用。
Biochem Int. 1991 May;24(1):187-97.
10
Identification and biochemical properties of 10-formyldihydrofolate, a novel folate found in methotrexate-treated cells.10-甲酰二氢叶酸的鉴定及其生化特性，一种在甲氨蝶呤处理的细胞中发现的新型叶酸。
J Biol Chem. 1988 May 25;263(15):7105-11.

引用本文的文献

1
Potential Drug-Nutrient Interactions of 45 Vitamins, Minerals, Trace Elements, and Associated Dietary Compounds with Acetylsalicylic Acid and Warfarin-A Review of the Literature.45 种维生素、矿物质、微量元素及相关膳食化合物与乙酰水杨酸和华法林的潜在药物-营养素相互作用：文献综述。
Nutrients. 2024 Mar 26;16(7):950. doi: 10.3390/nu16070950.
2
Decoding a cryptic mechanism of metronidazole resistance among globally disseminated fluoroquinolone-resistant Clostridioides difficile.解析全球流行的氟喹诺酮耐药艰难梭菌中甲硝唑耐药的神秘机制
Nat Commun. 2023 Jul 12;14(1):4130. doi: 10.1038/s41467-023-39429-x.
3
Cecal Metabolomic Fingerprint of Unscathed Rats: Does It Reflect the Good Response to a Provocative Decompression?未受损伤大鼠的盲肠代谢组学指纹图谱：它是否反映了对激发性减压的良好反应？
Front Physiol. 2022 May 17;13:882944. doi: 10.3389/fphys.2022.882944. eCollection 2022.
4
Phagocytosis and Epithelial Cell Invasion by Crohn's Disease-Associated Adherent-Invasive Are Inhibited by the Anti-inflammatory Drug 6-Mercaptopurine.与克罗恩病相关的黏附侵袭性细菌的吞噬作用和上皮细胞侵袭受到抗炎药物6-巯基嘌呤的抑制。
Front Microbiol. 2018 May 14;9:964. doi: 10.3389/fmicb.2018.00964. eCollection 2018.
5
Cofactor role for 10-formyldihydrofolic acid.10-甲酰基二氢叶酸的辅助因子作用。
Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):1031-6. doi: 10.1042/bj3081031.
6
Inhibition of folate-dependent enzymes by non-steroidal anti-inflammatory drugs.
Biochem J. 1992 Feb 15;282 ( Pt 1)(Pt 1):197-202. doi: 10.1042/bj2820197.

本文引用的文献

1
The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system.动物组织中脱氧核糖核酸的荧光测定，特别涉及中枢神经系统。
J Biol Chem. 1958 Jul;233(1):184-8.
2
Biosynthesis of the purines. XVIII. 5-Amino-1-ribosyl-4-imidazolecarboxamide 5'-phosphate transformylase and inosinicase.嘌呤的生物合成。十八。5-氨基-1-核糖基-4-咪唑甲酰胺5'-磷酸转甲酰酶和肌苷酶。
J Biol Chem. 1957 Dec;229(2):603-12.
3
Determination of three different pools of reduced one-carbon-substituted folates. 1. A study of the fundamental chemical reactions.三种不同还原型一碳取代叶酸池的测定。1. 基本化学反应的研究。
Anal Biochem. 1980 Nov 15;109(1):167-84. doi: 10.1016/0003-2697(80)90026-3.
4
An easy method for the determination of initial rates.一种测定初始速率的简便方法。
Biochem J. 1981 Mar 1;193(3):1009-12. doi: 10.1042/bj1931009.
5
Metabolism of 5-amino-4-imidazolecarboxamide riboside in cardiac and skeletal muscle. Effects on purine nucleotide synthesis.5-氨基-4-咪唑甲酰胺核苷在心肌和骨骼肌中的代谢。对嘌呤核苷酸合成的影响。
J Biol Chem. 1982 Sep 10;257(17):10178-83.
6
De novo purine synthesis in human lymphocytes. Partial co-purification of the enzymes and some properties of the pathway.人淋巴细胞中的嘌呤从头合成。酶的部分共纯化及该途径的一些特性。
J Biol Chem. 1983 Feb 10;258(3):1851-6.
7
Etiopathogenesis of murine SLE.小鼠系统性红斑狼疮的病因发病机制。
Immunol Rev. 1981;55:179-216. doi: 10.1111/j.1600-065x.1981.tb00343.x.
8
Antifolate actions of sulfasalazine on intact lymphocytes.柳氮磺吡啶对完整淋巴细胞的抗叶酸作用。
J Lab Clin Med. 1981 Jun;97(6):779-84.
9
Intestinal absorption and malabsorption of folates.叶酸的肠道吸收与吸收不良
Annu Rev Med. 1980;31:79-87. doi: 10.1146/annurev.me.31.020180.000455.
10
Inhibition of adenylosuccinate synthetase and adenylosuccinate lyase from Ehrlich ascites-tumour cells by 6-thioinosine 5'-phosphate.6-硫代肌苷5'-磷酸对艾氏腹水瘤细胞中腺苷酸琥珀酸合成酶和腺苷酸琥珀酸裂解酶的抑制作用
Biochem J. 1964 Aug;92(2):398-404. doi: 10.1042/bj0920398.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验