一种测定初始速率的简便方法。
An easy method for the determination of initial rates.
作者信息
Waley S G
出版信息
Biochem J. 1981 Mar 1;193(3):1009-12. doi: 10.1042/bj1931009.
Abstract
When the Michaelis-Menten equation is obeyed, the rate near the beginning of an enzyme-catalyzed reaction (or of an experiment on transport) can be found accurately from the slope of a chord joining two points on the progress curve. This slope gives the rate at an intermediate concentration. Exact values of this intermediate concentration are easily calculated from equations in the text, and a number of values have also been tabulated. Methods of using two chords to find the initial rate are given. A mid-point formula for numerical differentiation is advocated when the Michaelis-Menten equation does not hold.
摘要
当遵循米氏方程时，酶催化反应（或转运实验）开始阶段的速率可通过连接进程曲线上两点的弦的斜率准确得出。该斜率给出了中间浓度下的速率。此中间浓度的精确值可根据文中的方程轻松计算得出，并且也已制成表格列出。文中给出了使用两条弦来求初始速率的方法。当米氏方程不成立时，提倡使用数值微分的中点公式。
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Use of progress curves to investigate product inhibition in enzyme-catalysed reactions. Application to the soluble mitochondrial adenosine triphosphatase.利用进程曲线研究酶催化反应中的产物抑制作用。应用于可溶性线粒体三磷酸腺苷酶。
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