Ministry of Education & Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
Biosens Bioelectron. 2012 Oct-Dec;38(1):86-93. doi: 10.1016/j.bios.2012.05.006. Epub 2012 May 24.
A facile and feasible magneto-controlled immunosensing platform was designed for sensitive electrochemical immunoassay of brevetoxin B (BTX-2) in seafood by using guanine-assembled graphene nanoribbons (GGNRs) as molecular tags on a home-made magnetic carbon paste electrode. Initially, monoclonal mouse anti-BTX-2 antibodies were covalently immobilized on the surface of magnetic beads, which were used as the immunosensing probes for the capture of BTX-2. The recognition elements were prepared by chemical modification of bovine serum albumin-BTX-2 conjugates (BTX-2-BSA) with the GGNRs. Based on a competitive-type immunoassay format, the formed magnetic immunocomplex was integrated on the electrode with an external magnet, followed by determination in pH 6.5 phosphate-buffered solution containing 2 μM Ru(bpy)(3)Cl(2). Under optimal conditions, the electrochemical signals decreased with the increasing BTX-2 concentrations in the sample. The dynamic concentration range spanned from 1.0 pg mL(-1) to 10 ng mL(-1) with a detection limit of 1.0 pg mL(-1) BTX-2. Inter- and intra-batch assay precisions were substantially improved by resorting to the GGNR manifold. The method featured unbiased identification of negative (blank) and positive samples. No significant differences at the 95% confidence level were encountered in the analysis of 12 spiked samples including S. constricta, M. senhousia and T. granosa between the electrochemical immunoassay and commercially available enzyme-linked immunosorbent assay (ELISA) for determination of BTX-2.
设计了一种简便可行的磁控免疫传感平台，用于通过使用鸟嘌呤组装的石墨烯纳米带（GGNRs）作为分子标记物，在自制的磁性碳糊电极上对海鲜中的布雷毒素 B（BTX-2）进行灵敏的电化学免疫分析。最初，单克隆抗 BTX-2 抗体被共价固定在磁性珠的表面上，用作捕获 BTX-2 的免疫传感探针。识别元件是通过牛血清白蛋白-BTX-2 缀合物（BTX-2-BSA）与 GGNRs 的化学修饰制备的。基于竞争型免疫分析格式，形成的磁性免疫复合物在外加磁场的作用下被集成在电极上，随后在含有 2 μM Ru（bpy）（3）Cl（2）的 pH 6.5 磷酸盐缓冲溶液中进行测定。在最佳条件下，电化学信号随样品中 BTX-2 浓度的增加而降低。动态浓度范围从 1.0 pg mL（-1）到 10 ng mL（-1），检测限为 1.0 pg mL（-1）BTX-2。通过采用 GGNR 歧管，可以大大提高批间和批内测定的精密度。该方法具有对阴性（空白）和阳性样品进行无偏识别的特点。在分析 12 个包括 S. constricta、M. senhousia 和 T. granosa 在内的加标样品时，电化学免疫分析与商业酶联免疫吸附测定（ELISA）之间在 BTX-2 的测定中未发现 95%置信水平的显著差异。
