Pujades C, Bastos R, Enrich C, Bachs O
Department of Cell Biology, Faculty of Medicine, University of Barcelona, Spain.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):1287-91. doi: 10.1016/s0006-291x(05)80926-x.
After proliferative activation of rat liver cells in vivo by a partial hepatectomy a decrease of the calmodulin content in the three plasma membrane domains (blood sinusoidal, canalicular and lateral) was observed. At 24 hours after partial hepatectomy calmodulin was found to be 3 fold lower in the sinusoidal and lateral fractions whereas a 2 fold decrease was detected in the canalicular domain. Decreases on the actin levels have been also detected at 24 hours after a partial hepatectomy. Since at this time after surgery increases on nuclear actin and calmodulin have been reported, these results suggest the possibility that the actin and calmodulin dissociated from the plasma membrane after a partial hepatectomy could subsequently be translocated into the nuclei.
通过部分肝切除术在体内对大鼠肝细胞进行增殖激活后，观察到三个质膜结构域（血窦、胆小管和侧面）中的钙调蛋白含量降低。在部分肝切除术后24小时，发现血窦和侧面部分的钙调蛋白降低了3倍，而胆小管结构域中检测到降低了2倍。在部分肝切除术后24小时也检测到肌动蛋白水平的降低。由于在手术后的这个时候已经报道了核肌动蛋白和钙调蛋白的增加，这些结果表明部分肝切除术后从质膜解离的肌动蛋白和钙调蛋白随后可能转移到细胞核中的可能性。
