Simon H, Wenzelides K, Guski H, Kranz D
Zentralbl Allg Pathol. 1985;130(1):57-62.
Partial hepatectomy (30 percent of the liver weight) was performed in 225 female Wistar rats, ages 6 weeks, 6 and 9 months. The animals were sacrificed in intervals between 4 hours and 21 d after the operation. The automated microscopic image analysis after Simon et al. (1984) and histoautoradiography were utilized for evaluation of liver cell regeneration. Despite measurement of more than 1,000 cells per animal we were not able to confirm enlargement of hepatocytes as the first reaction to partial hepatectomy The number of liver cells per unit area increased whereas sinusoidal size remained rather constant, corresponding to a decrease in average cell size and in the standard deviation of cell area. The importance of binucleated hepatocytes in the process is emphasized. Measurement of the nucleoli and of the mean extinction values of liver cell nuclei as a measure of the DNA content did not provide any essentially new perspectives. The investigation did however emphasize the importance of the extent of the hepatic resection and the age of the experimental animals on the intensity and subsequent course of liver cell regeneration. Proliferation of the sinusoidal cells themselves after partial hepatectomy could not definitively be demonstrated to be age-dependent.
对225只6周龄、6月龄和9月龄的雌性Wistar大鼠进行了部分肝切除术（切除肝脏重量的30%）。术后4小时至21天期间间隔处死动物。采用Simon等人（1984年）的自动显微图像分析和组织放射自显影术评估肝细胞再生。尽管每只动物测量了1000多个细胞，但我们无法证实肝细胞增大是对部分肝切除术的第一反应。单位面积的肝细胞数量增加，而肝血窦大小保持相当恒定，这与平均细胞大小和细胞面积标准差的降低相对应。强调了双核肝细胞在该过程中的重要性。测量核仁和肝细胞细胞核的平均消光值作为DNA含量的指标并未提供任何本质上新的观点。然而，该研究强调了肝切除范围和实验动物年龄对肝细胞再生强度及后续进程的重要性。部分肝切除术后肝血窦细胞自身的增殖不能明确证明与年龄有关。
