Inoue K, Akita N, Yamashita S, Shiba T, Fujita T
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):1311-6. doi: 10.1016/s0006-291x(05)80930-1.
Activities of heterologous promoters and enhancers in cultured rainbow trout liver cells were examined employing the bacterial chloramphenicol acetyltransferase gene as the reporter. SV40 promoter-enhancer and Rous sarcoma virus long terminal repeat directed constitutive expression at high levels. Moloney murine leukemia virus long terminal repeat and SV40 promoter combined with Adenovirus type 2 enhancer were also constitutively expressed. Drosophila Hsp70 promoter was activated when the transformed cells were cultured at 25 degrees C, a higher temperature than the temperature normally used, in faithful response to heat shock.
