State Key Laboratory of Organic Geochemistry, CAS Centre for Pearl River Delta Environmental Pollution and Control Research, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
Environ Pollut. 2012 Oct;169:42-9. doi: 10.1016/j.envpol.2012.05.007. Epub 2012 Jun 7.
Antibiotic susceptibility, detection of sul gene types and presence of class 1, 2 and 3 integrons and gene cassettes using PCR assays were investigated in 3456 Escherichia coli isolates obtained from 38 sampling sites of the Dongjiang River catchment in the dry and wet seasons. 89.1% of the isolates were resistant and 87.5% showed resistance to at least three antibiotics. sul2 was detected most frequently in 89.2% of 1403 SXT-resistant isolates. The presence of integrons (class 1 and 2) was frequently observed (82.3%) while no class 3 integron was found. In these integrons, 21 resistance genes of 14 gene cassette arrays and 10 different families of resistance genes were identified. Three gene cassette arrays, aac(6')-Ib-cr-aar-3-dfrA27-aadA16, aacA4-catB3-dfrA1 and aadA2-lnuF, were detected for the first time in surface water. The results showed that bacterial resistance in the catchment was seriously influenced by human activities, especially discharge of wastewater.
采用 PCR 法检测了从东江流域 38 个采样点采集的旱、雨季 3456 株大肠埃希菌的药敏性、sul 基因类型、1 类、2 类和 3 类整合子及基因盒的存在情况。89.1%的分离株耐药，87.5%的分离株至少对 3 种抗生素耐药。在 1403 株 SXT 耐药分离株中，sul2 检出率最高（89.2%）。整合子（1 类和 2 类）的存在率较高（82.3%），但未发现 3 类整合子。在这些整合子中，鉴定出了 14 个基因盒数组中的 21 个耐药基因和 10 个不同家族的耐药基因。首次在地表水中检测到 3 个基因盒数组 aac(6')-Ib-cr-aar-3-dfrA27-aadA16、aacA4-catB3-dfrA1 和 aadA2-lnuF。结果表明，流域内细菌耐药性受到人类活动的严重影响，尤其是污水排放的影响。
