Department of Experimental Zoology and Neurobiology, Institute of Biology, University of Pecs, 7624 Pecs, Hungary.
J Mol Histol. 2012 Oct;43(5):565-9. doi: 10.1007/s10735-012-9426-y. Epub 2012 Jun 9.
Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non- and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.
自 20 世纪 60 年代以来，挥发性麻醉剂的神经保护特性已得到认可。然而，关于麻醉药物预处理的潜在视网膜保护作用知之甚少。视网膜缺血可以通过永久性双侧颈总动脉闭塞（BCCAO）来模拟。在这里，我们研究了七氟醚预处理对大鼠视网膜缺血损伤的程度。在 BCCAO 手术和预处理期间，Wistar 大鼠用 1 MAC 的七氟醚麻醉。麻醉箱中的氧气、二氧化碳和麻醉蒸气浓度用气体分析仪进行监测。我们检查了 4 组：非预处理和预处理组，分别在对照和 BCCAO 动物中。预处理期持续 1 小时，在 BCCAO 前 1 天进行。在 2 周存活后，对视网膜进行组织学评估，以确定节细胞层中的细胞数量以及整个视网膜和所有视网膜层的厚度。七氟醚预处理可减轻 BCCAO 诱导的视网膜缺血损伤。与非预处理组相比，BCCAO 后预处理动物的视网膜厚度和节细胞层中的细胞数量保留更多。这些结果表明，七氟醚预处理可能为视网膜保护策略提供新的视角。
