Iliescu Daniela Adriana, Ciubotaru Alexandra, Ghiţă Mihai Aurelian, Dumitru Adrian, Zăgrean Leon
Physiology Department, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Ophthalmology Department, "Dr. Carol Davila" Central Military University Emergency Hospital, Bucharest, Romania.
Rom J Ophthalmol. 2018 Jan-Mar;62(1):24-33.
Hyperglycemia and bright light are powerful stress agents that produce an enhanced retinal damage, when simultaneously acting on retina. Previous studies have shown that preconditioning with sevoflurane anesthesia offers a certain degree of protection to retinal cells against light damage. The objective of this study was to explore the effect of sevoflurane anesthetic preconditioning on a model of light-induced retinal degeneration in diabetic rats. Wistar rats that were randomly divided into four groups: control (rats exposed to photostress), group 1 (rats exposed to photostress and sevoflurane preconditioning), group 2 (diabetic rats exposed to photostress), group 3 (diabetic rats exposed to photostress and sevoflurane preconditioning) were used for this experiment. We recorded basal electroretinogram (ERG), at 36 h and 14 days after photostress and performed histological analysis of the retina. Results showed that sevoflurane has a protective effect on light-induced neuroretinal degeneration proved by significantly less variations of the ERG before and after photostress. Diabetes appears to increase the damaging effect of photostress on retina and attenuate the protection provided by sevoflurane preconditioning.
高血糖和强光都是强大的应激因素，当它们同时作用于视网膜时，会加剧视网膜损伤。先前的研究表明，七氟醚麻醉预处理可为视网膜细胞提供一定程度的光损伤保护。本研究的目的是探讨七氟醚麻醉预处理对糖尿病大鼠光诱导视网膜变性模型的影响。将Wistar大鼠随机分为四组：对照组（暴露于光应激的大鼠）、第1组（暴露于光应激并进行七氟醚预处理的大鼠）、第2组（糖尿病且暴露于光应激的大鼠）、第3组（糖尿病且暴露于光应激并进行七氟醚预处理的大鼠）用于本实验。我们记录了光应激后36小时和14天的基础视网膜电图（ERG），并对视网膜进行了组织学分析。结果表明，七氟醚对光诱导的神经视网膜变性具有保护作用，光应激前后ERG的变化明显较小即证明了这一点。糖尿病似乎会增加光应激对视网膜的损伤作用，并减弱七氟醚预处理提供的保护作用。
