Department of Forest Microbiology, Forestry and Forest Products Research Institute, Tsukuba, lbaraki, Japan.
Mycologia. 2012 Nov-Dec;104(6):1369-80. doi: 10.3852/12-068. Epub 2012 Jun 8.
Tricholoma matsutake (S. Ito & S. Imai) Singer and its allied species are referred to as matsutake worldwide and are the most economically important edible mushrooms in Japan. They are widely distributed in the northern hemisphere and established an ectomycorrhizal relationship with conifer and broadleaf trees. To clarify relationships among T. matsutake and its allies, and to delimit phylogenetic species, we analyzed multilocus datasets (ITS, megB1, tef, gpd) with samples that were correctly identified based on morphological characteristics. Phylogenetic analyses clearly identified four major groups: matsutake, T. bakamatsutake, T. fulvocastaneum and T. caligatum; the latter three species were outside the matsutake group. The haplotype analyses and median-joining haplotype network analyses showed that the matsutake group included four closely related but clearly distinct taxa (T. matsutake, T. anatolicum, Tricholoma sp. from Mexico and T. magnivelare) from different geographical regions; these were considered to be distinct phylogenetic species.
松茸（S. Ito & S. Imai）及其相关物种在世界范围内被称为松茸，是日本最重要的经济食用蘑菇。它们广泛分布于北半球，与针叶树和阔叶树建立了外生菌根关系。为了阐明松茸及其相关物种之间的关系，并对其进行系统发育种的划分，我们对基于形态特征正确鉴定的样本进行了多基因座数据集（ITS、megB1、tef、gpd）分析。系统发育分析清楚地确定了四个主要群体：松茸、T. bakamatsutake、T. fulvocastaneum 和 T. caligatum；后三个物种不在松茸群体内。单倍型分析和中位数连接单倍型网络分析表明，松茸群体包括四个密切相关但明显不同的分类群（来自不同地理区域的 T. matsutake、T. anatolicum、来自墨西哥的 Tricholoma sp. 和 T. magnivelare）；这些被认为是不同的系统发育种。
