Aoki Wataru, Bergius Niclas, Kozlan Serhii, Fukuzawa Fuminori, Okuda Hitomi, Murata Hitoshi, Ishida Takahide A, Vaario Lu-Min, Kobayashi Hisayasu, Kalmiş Erbil, Fukiharu Toshimitsu, Gisusi Seiki, Matsushima Ken-Ichi, Terashima Yoshie, Narimatsu Maki, Matsushita Norihisa, Ka Kang-Hyeon, Yu Fuqiang, Yamanaka Takashi, Fukuda Masaki, Yamada Akiyoshi
a Department of Agriculture, Graduate School of Science and Technology, Shinshu University.
b Länsstyrelsen i Västmanlands län.
Mycoscience. 2022 Sep 30;63(5):197-214. doi: 10.47371/mycosci.2022.07.004. eCollection 2022.
Matsutake mushrooms are among the best-known edible wild mushroom taxa worldwide. The representative is from East Asia and the northern and central regions of Europe. Here, we report the existence of under fir trees in Eastern Europe (i.e., Ukraine), as confirmed by phylogenetic analysis of nine loci on the nuclear and mitochondrial genomes. All specimens from Japan, Bhutan, China, North Korea, South Korea, Sweden, Finland, and Ukraine formed a clade according to the phylogeny of the internal transcribed spacer region. The European population of was clustered based on the β2 tubulin gene, with a moderate bootstrap value. In contrast, based on analyses of three loci, i.e., 2, 1, and the β2 tubulin gene, specimens sampled from Bhutan and China belonged to a clade independent of the other specimens of this species, implying a genetically isolated population. As biologically available type specimens of have not been designated since its description as a new species from Japan in 1925, we established an epitype of this fungus, sampled in a forest in Nagano, Japan.
松口蘑是全球最知名的可食用野生蘑菇类群之一。其代表性种类来自东亚以及欧洲北部和中部地区。在此，我们报告在东欧（即乌克兰）的冷杉树下存在松口蘑，这通过对核基因组和线粒体基因组上九个位点的系统发育分析得以证实。根据内部转录间隔区的系统发育，来自日本、不丹、中国、朝鲜、韩国、瑞典、芬兰和乌克兰的所有标本形成了一个进化枝。欧洲的松口蘑种群基于β2微管蛋白基因聚类，自展值适中。相比之下，基于对三个位点（即2、1和β2微管蛋白基因）的分析，从不丹和中国采集的松口蘑标本属于一个独立于该物种其他标本的进化枝，这意味着存在一个基因隔离的种群。由于自1925年松口蘑从日本被描述为新物种以来，尚未指定具有生物学可用性的模式标本，我们在日本长野的一个松口蘑森林中采集样本，建立了该真菌的一个后选模式标本。
