State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
Plant Cell Rep. 2012 Oct;31(10):1823-8. doi: 10.1007/s00299-012-1295-z. Epub 2012 Jun 9.
Garlic is widely used as a spice throughout the world for the culinary value of its flavor and aroma, which are created by the chemical transformation of a series of organic sulfur compounds. To analyze the transcriptome of Allium sativum and discover the genes involved in sulfur metabolism, cDNAs derived from the total RNA of Allium sativum buds were analyzed by Illumina sequencing. Approximately 26.67 million 90 bp paired-end clean reads were achieved in two libraries. A total of 127,933 unigenes were generated by de novo assembly and were compared with the sequences in public databases. Of these, 45,286 unigenes had significant hits to the sequences in the Nr database, 29,514 showed significant similarity to known proteins in the Swiss-Prot database and, 20,706 and 21,952 unigenes had significant similarity to existing sequences in the KEGG and COG databases, respectively. Moreover, genes involved in organic sulfur biosynthesis were identified. These unigenes data will provide the foundation for research on gene expression, genomics and functional genomics in Allium sativum. Key message The obtained unigenes will provide the foundation for research on functional genomics in Allium sativum and its closely related species, and fill the gap of the existing plant EST database.
大蒜作为一种香料在全世界范围内广泛使用，其风味和香气是由一系列有机硫化合物的化学转化而产生的。为了分析大蒜的转录组并发现参与硫代谢的基因，我们对大蒜芽的总 RNA 进行了 cDNA 分析，使用 Illumina 测序进行分析。在两个文库中获得了约 2667 万对 90bp 末端清洁读数。通过从头组装生成了 127933 个非编码 RNA，并与公共数据库中的序列进行了比较。其中，45286 个非编码 RNA 与 Nr 数据库中的序列有显著的匹配，29514 个非编码 RNA 与 Swiss-Prot 数据库中已知的蛋白质有显著的相似性，20706 和 21952 个非编码 RNA 与 KEGG 和 COG 数据库中的现有序列有显著的相似性。此外，还鉴定出了参与有机硫生物合成的基因。这些非编码 RNA 数据将为大蒜及其近缘种的基因表达、基因组学和功能基因组学研究提供基础。
关键词：大蒜；转录组；硫代谢；有机硫生物合成；非编码 RNA
