Erwall C, Friberg U, Bagger-Sjöbäck D, Rask-Andersen H
Department of Otolaryngology, University Hospital, Uppsala, Sweden.
ORL J Otorhinolaryngol Relat Spec. 1988;50(1):42-53. doi: 10.1159/000275968.
The acute and chronic effects of treatment with ethacrynic acid (EA) and furosemide (FU) on the structure of the murine endolymphatic sac were studied by means of light and transmission electron microscopy. The animals were treated with loop diuretics in doses which are known to cause morphological alterations of the stria vascularis and a significant reduction of the endocochlear potential. A single intravenous injection of either EA or FU resulted in immediate morphological changes such as an increase in cytoplasmic contents of endoplasmic reticulum and more prominent Golgi structures of the light cells. These cells developed membrane-bound granules and a smooth tubular network in the apical cytoplasm. These findings together with the appearance of a precipitate on the luminal aspect of the cell membrane suggested secretory activity. Ten days after daily intraperitoneal injections with loop diuretics in subtoxic doses, the epithelial cytoarchitecture of the endolymphatic sac was altered, with pronounced veiling of the light cells by the dark cells. It is concluded that the changes in the endolymphatic sac epithelium after treatment with ototoxic diuretics may not be a result of a primary toxic effect on the sac per se, but rather be secondary to alterations in fluid and ion homeostasis in the rest of the inner ear.
通过光学显微镜和透射电子显微镜研究了依他尼酸（EA）和呋塞米（FU）治疗对小鼠内淋巴囊结构的急性和慢性影响。给动物使用已知会导致血管纹形态改变和内耳蜗电位显著降低的剂量的袢利尿剂。单次静脉注射EA或FU会立即导致形态学变化，如内质网的细胞质内容物增加以及亮细胞的高尔基体结构更明显。这些细胞在顶端细胞质中形成膜结合颗粒和平滑的管状网络。这些发现以及细胞膜腔面出现沉淀物表明有分泌活性。每天腹腔注射亚毒性剂量的袢利尿剂十天后，内淋巴囊的上皮细胞结构发生改变，暗细胞明显遮盖亮细胞。得出的结论是，耳毒性利尿剂治疗后内淋巴囊上皮的变化可能不是对囊本身的原发性毒性作用的结果，而是继发于内耳其他部位的液体和离子稳态的改变。
