用于实时多色跟踪动态生物系统的 DNA 多荧光团
DNA polyfluorophores for real-time multicolor tracking of dynamic biological systems.
机构信息
Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
出版信息
Angew Chem Int Ed Engl. 2012 Jul 16;51(29):7176-80. doi: 10.1002/anie.201201928. Epub 2012 Jun 8.
Abstract
Dye-ing to live: Spectral limitations of common organic dyes make it difficult or impossible to visualize and follow multiple biological components in rapidly moving systems. The development of a multispectral set of improved DNA-scaffolded fluorophores is described. Their use in multicolor cellular imaging (see scheme) and in tracking of biological motions on the subsecond timescale is demonstrated.
摘要
染色求生
常见有机染料的光谱限制使得在快速移动的系统中难以或不可能对多个生物组分进行可视化和跟踪。本文描述了一组多光谱改良 DNA 支架荧光团的开发。展示了它们在多色细胞成像（见方案）和亚秒级生物运动跟踪中的应用。
相似文献
1
DNA polyfluorophores for real-time multicolor tracking of dynamic biological systems.用于实时多色跟踪动态生物系统的 DNA 多荧光团
Angew Chem Int Ed Engl. 2012 Jul 16;51(29):7176-80. doi: 10.1002/anie.201201928. Epub 2012 Jun 8.
2
Polyfluorophores on a DNA backbone: a multicolor set of labels excited at one wavelength.DNA主链上的多荧光团：一组在单一波长激发的多色标记。
J Am Chem Soc. 2009 Mar 25;131(11):3923-33. doi: 10.1021/ja805502k.
3
Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging.多色标记抗体与 DNA 主链上的多荧光团的应用于细胞成像。
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3493-8. doi: 10.1073/pnas.1017349108. Epub 2011 Feb 14.
4
A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy.一种通过荧光显微镜检测单个活的人类细胞中DNA纳米结构的实用方法。
Methods. 2014 May 15;67(2):185-92. doi: 10.1016/j.ymeth.2014.01.009. Epub 2014 Jan 17.
5
Genetically encoded multispectral labeling of proteins with polyfluorophores on a DNA backbone.在 DNA 主链上用多聚荧光团对蛋白质进行基因编码的多光谱标记。
J Am Chem Soc. 2013 Apr 24;135(16):6184-91. doi: 10.1021/ja4004393. Epub 2013 Apr 16.
6
Serial imaging of micro-agents and cancer cell spheroids in a microfluidic channel using multicolor fluorescence microscopy.使用多色荧光显微镜对微流控通道中的微试剂和癌细胞球体进行连续成像。
PLoS One. 2021 Jun 15;16(6):e0253222. doi: 10.1371/journal.pone.0253222. eCollection 2021.
7
Fast, Background-Free DNA-PAINT Imaging Using FRET-Based Probes.基于荧光共振能量转移的探针的快速、无背景 DNA-PAINT 成像。
Nano Lett. 2017 Oct 11;17(10):6428-6434. doi: 10.1021/acs.nanolett.7b03425. Epub 2017 Sep 21.
8
Visualization of micro-agents and surroundings by real-time multicolor fluorescence microscopy.实时多色荧光显微镜观察微试剂和周围环境。
Sci Rep. 2022 Aug 4;12(1):13375. doi: 10.1038/s41598-022-17297-7.
9
A biphenyl type two-photon fluorescence probe for monitoring the mitochondrial membrane potential.一种用于监测线粒体膜电位的联苯型双光子荧光探针。
Cell Struct Funct. 2014;39(2):125-33. doi: 10.1247/csf.14006. Epub 2014 Oct 16.
10
Tracking single particles for hours via continuous DNA-mediated fluorophore exchange.通过连续 DNA 介导的荧光团交换，实现对单个粒子的连续数小时追踪。
Nat Commun. 2021 Jul 21;12(1):4432. doi: 10.1038/s41467-021-24223-4.
引用本文的文献
1
Ultrasensitive and Multiplexed Protein Imaging with Clickable and Cleavable Fluorophores.通过点击反应和可裂解荧光染料实现超高灵敏和多重化的蛋白质成像。
Anal Chem. 2024 May 7;96(18):7281-7288. doi: 10.1021/acs.analchem.4c01273. Epub 2024 Apr 25.
2
Ultrasensitive and multiplexed protein imaging with clickable and cleavable fluorophores.使用可点击和可切割荧光团进行超灵敏和多重蛋白质成像。
bioRxiv. 2023 Oct 23:2023.10.20.563323. doi: 10.1101/2023.10.20.563323.
3
Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization.通过连续杂交实现高多重化的单细胞原位 RNA 和 DNA 分析。
Molecules. 2020 Oct 23;25(21):4900. doi: 10.3390/molecules25214900.
4
Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase.响应性荧光核苷酸可作为有效底物，用于探测末端尿苷酰转移酶。
Chem Commun (Camb). 2020 Oct 21;56(82):12319-12322. doi: 10.1039/d0cc05092j. Epub 2020 Sep 17.
5
Single-Cell RNA Analysis With Switchable Fluorescent Oligonucleotides.利用可切换荧光寡核苷酸进行单细胞RNA分析
Front Cell Dev Biol. 2018 Apr 11;6:42. doi: 10.3389/fcell.2018.00042. eCollection 2018.
6
Luminescent Carbon Dot Mimics Assembled on DNA.基于 DNA 组装的上转换发光碳点模拟物
J Am Chem Soc. 2017 Sep 20;139(37):13147-13155. doi: 10.1021/jacs.7b07420. Epub 2017 Sep 12.
7
Color-Change Photoswitching of an Alkynylpyrene Excimer Dye.炔基芘二聚体染料的颜色变化光致开关。
Angew Chem Int Ed Engl. 2017 Jun 1;56(23):6497-6501. doi: 10.1002/anie.201701235. Epub 2017 May 5.
8
Efficient synthesis of fluorescent alkynyl C-nucleosides via Sonogashira coupling for the preparation of DNA-based polyfluorophores.通过Sonogashira偶联高效合成荧光炔基C-核苷用于制备基于DNA的多荧光团。
Org Biomol Chem. 2016 Jul 6;14(27):6407-12. doi: 10.1039/c6ob01199c.
9
Pattern-Based Detection of Anion Pollutants in Water with DNA Polyfluorophores.基于模式的DNA多氟荧光团检测水中阴离子污染物
Chem Sci. 2015 Apr 1;6(4):2575-2583. doi: 10.1039/C4SC03992K.
10
In Vitro Fluorogenic Real-Time Assay of the Repair of Oxidative DNA Damage.氧化DNA损伤修复的体外荧光实时检测
Chembiochem. 2015 Jul 27;16(11):1637-46. doi: 10.1002/cbic.201500184. Epub 2015 Jun 12.
本文引用的文献
1
A DNA-based light-harvesting antenna.一种基于DNA的光捕获天线。
Angew Chem Int Ed Engl. 2012 Jan 23;51(4):916-9. doi: 10.1002/anie.201103295. Epub 2011 Dec 8.
2
Intravital imaging.活体成像。
Cell. 2011 Nov 23;147(5):983-91. doi: 10.1016/j.cell.2011.11.004.
3
Two-color STED microscopy of living synapses using a single laser-beam pair.双色 STED 显微镜在单个激光束对下对活突触进行成像。
Biophys J. 2011 Nov 16;101(10):2545-52. doi: 10.1016/j.bpj.2011.10.011. Epub 2011 Nov 15.
4
Fluorescent DNA nanotags featuring covalently attached intercalating dyes: synthesis, antibody conjugation, and intracellular imaging.具有共价连接的嵌入染料的荧光 DNA 纳米标签：合成、抗体缀合和细胞内成像。
Bioconjug Chem. 2011 Aug 17;22(8):1491-502. doi: 10.1021/bc100485f. Epub 2011 Jul 22.
5
Diversity-driven chemical probe development for biomolecules: beyond hypothesis-driven approach.基于多样性的生物分子化学探针开发：超越假设驱动方法。
Chem Soc Rev. 2011 Jul;40(7):3613-26. doi: 10.1039/c0cs00172d. Epub 2011 Apr 27.
6
Chemistry of nucleic acids: impacts in multiple fields.核酸化学：多领域的影响。
Chem Commun (Camb). 2011 Jul 7;47(25):7018-24. doi: 10.1039/c1cc11021g. Epub 2011 Apr 11.
7
Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging.多色标记抗体与 DNA 主链上的多荧光团的应用于细胞成像。
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3493-8. doi: 10.1073/pnas.1017349108. Epub 2011 Feb 14.
8
Fluorescent DNA-based enzyme sensors.荧光 DNA 酶传感器。
Chem Soc Rev. 2011 Dec;40(12):5756-70. doi: 10.1039/c0cs00162g. Epub 2011 Feb 2.
9
Quantum dots brighten biological imaging.量子点照亮生物成像。
Prog Histochem Cytochem. 2011 Feb;45(4):201-37. doi: 10.1016/j.proghi.2010.11.001. Epub 2010 Dec 31.
10
Visualizing enveloping layer glycans during zebrafish early embryogenesis.可视化斑马鱼早期胚胎发生过程中的被膜层聚糖。
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10360-5. doi: 10.1073/pnas.0912081107. Epub 2010 May 20.
Zotero 插件