Yasui Takao, Reza Mohamadi Mohamad, Kaji Noritada, Okamoto Yukihiro, Tokeshi Manabu, Baba Yoshinobu
Biomicrofluidics. 2011 Dec;5(4):44114-441149. doi: 10.1063/1.3668233. Epub 2011 Dec 12.
In this paper, we study characteristics of polymers (methylcellulose, hypromellose ((hydroxypropyl)methyl cellulose), poly(vinylpyrrolidone), and poly(vinyl alcohol)) with different chemical structures for microchip electrophoresis of non-denatured protein samples in a plastic microchip made of poly(methyl methacrylate) (PMMA). Coating efficiency of these polymers for controlling protein adsorption onto the channel surface of the plastic microchip, wettability of the PMMA surface, and electroosmotic flow in the PMMA microchannels in the presence of these polymers were compared. Also relative electrophoretic mobility of protein samples in solutions of these polymers was studied. We showed that when using low polymer concentrations (lower than the polymer entanglement point) where the sieving effect is substantially negligible, the interaction of the samples with the polymer affected the electrophoretic mobility of the samples. This effect can be used for achieving better resolution in microchip electrophoresis of protein samples.
在本文中，我们研究了具有不同化学结构的聚合物（甲基纤维素、羟丙基甲基纤维素、聚乙烯吡咯烷酮和聚乙烯醇）的特性，用于在由聚甲基丙烯酸甲酯（PMMA）制成的塑料微芯片中对非变性蛋白质样品进行微芯片电泳。比较了这些聚合物在控制蛋白质吸附到塑料微芯片通道表面的涂层效率、PMMA表面的润湿性以及在这些聚合物存在下PMMA微通道中的电渗流。此外，还研究了蛋白质样品在这些聚合物溶液中的相对电泳迁移率。我们表明，当使用低聚合物浓度（低于聚合物缠结点）时，筛分效应基本可以忽略不计，样品与聚合物的相互作用会影响样品的电泳迁移率。这种效应可用于在蛋白质样品的微芯片电泳中实现更好的分辨率。
