Fried A H
J Virol. 1972 Dec;10(6):1236-9. doi: 10.1128/JVI.10.6.1236-1239.1972.
Ribonucleic acid (RNA) was isolated from CV-1 cells 44 hr after simian virus 40 (SV40) infection. The molecules containing SV40 base sequences were characterized with respect to their buoyant density distribution. The density of these molecules was compared to that of single- and double-stranded RNA synthesized by using SV40 DNA and Escherichia coli RNA polymerase. The results suggest that about 20% of the SV40 RNA in the infected cells consisted of partially double-stranded molecules.
在猿猴病毒40(SV40)感染44小时后,从CV-1细胞中分离出核糖核酸(RNA)。对含有SV40碱基序列的分子进行了浮力密度分布特征分析。将这些分子的密度与使用SV40 DNA和大肠杆菌RNA聚合酶合成的单链和双链RNA的密度进行了比较。结果表明,受感染细胞中约20%的SV40 RNA由部分双链分子组成。