Laboratoire de biologie Cellulaire et Moléculaire, Faculté de Medecine Dentaire Monastir, Rue Avicenne, Monastir, 5000 Tunisia.
Cancer Cell Int. 2012 Jun 11;12(1):26. doi: 10.1186/1475-2867-12-26.
The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.
We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.
The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.
In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.
从黄连木果实中提取的双没食子酸（DGA），其抗增殖和促凋亡活性在人类淋巴母细胞 TK6 细胞上进行了研究。
我们试图描述 DGA 激活的凋亡途径。通过 DNA 片段化、PARP 切割和评估半胱天冬酶活性来检测凋亡。
从 DGA 的 8.5μg/ml 开始抑制淋巴母细胞的增殖。DNA 片段化和 PARP 切割证实了凋亡的诱导。我们已经证明，DGA 通过激活半胱天冬酶-8 外源性途径诱导细胞凋亡。半胱天冬酶-3 也呈剂量依赖性方式激活。
总之，DGA 在 TK6 细胞中表现出诱导凋亡的作用，这表明其具有作为癌症预防剂的潜力。
