Skała Ewa, Toma Monika, Kowalczyk Tomasz, Śliwiński Tomasz, Sitarek Przemysław
Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland.
Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland.
Cytotechnology. 2018 Dec;70(6):1585-1594. doi: 10.1007/s10616-018-0251-3. Epub 2018 Aug 31.
Rhaponticum carthamoides transformed root extract induces double strand DNA damage by increasing the number of phosphorylated H2A.X- and cleaved PARP1-positive U87MG cells and patient-derived IV grade glioma cells. Furthermore, treatment of these cells with root extract causes down-regulation of UHRF1 and DNMT1. Transformed root extract is rich in caffeoylquinic acid derivatives, especially tricaffeoylquinic acid derivatives. Our findings demonstrate that the R. carthamoides transformed root extract may trigger apoptosis in glioma cells by induction of DNA damage, PARP cleavage and epigenetic modification.
刺续断转化根提取物通过增加磷酸化H2A.X和裂解PARP1阳性的U87MG细胞以及患者来源的IV级神经胶质瘤细胞的数量来诱导双链DNA损伤。此外，用根提取物处理这些细胞会导致UHRF1和DNMT1的下调。转化根提取物富含咖啡酰奎宁酸衍生物，尤其是三咖啡酰奎宁酸衍生物。我们的研究结果表明，刺续断转化根提取物可能通过诱导DNA损伤、PARP裂解和表观遗传修饰来触发神经胶质瘤细胞的凋亡。
