Dipartimento di Biotecnologie per il Monitoraggio Agro-Alimentare ed Ambientale (BIOMAA), Università Mediterranea di Reggio Calabria, Facoltà di Agraria - Salita Melissari, I-89124 Reggio, Calabria, RC, Italy.
Nat Prod Res. 2013;27(10):880-7. doi: 10.1080/14786419.2012.691491. Epub 2012 Jun 12.
The aerial part of Artemisia arborescens L. (Asteraceae) was extracted with water and methanol, and both extracts were fractionated using n-hexane, chloroform, ethyl acetate and n-butanol. The potential phytotoxicity of both crude extracts and their fractions were assayed in vitro on seed germination and root growth of lettuce (Lactuca sativa L.), a sensitive species largely employed in the allelopathy studies. The inhibitory activities were analysed by dose-response curves and the ED 50 were estimated. Crude extracts strongly inhibited both germination and root growth processes. The fraction-bioassay indicated the following hierarchy of phytotoxicity for both physiological processes: ethyl acetate ≥ n-hexane > chloroform ≥ n-butanol. On the n-hexane fraction, GC-MS analyses were carried out to characterise and quantify some of the potential allelochemicals. Twenty-one compounds were identified and three of them, camphor, trans-caryophyllene and pulegone were quantified.
艾蒿（菊科）的地上部分用水和甲醇提取，并用正己烷、氯仿、乙酸乙酯和正丁醇对提取物进行了分级。在体外，用生菜（生菜）种子发芽和根生长来测定两种粗提取物及其级分的潜在植物毒性，生菜是化感研究中广泛使用的敏感物种。通过剂量反应曲线分析抑制活性，并估计 ED 50。粗提取物强烈抑制了发芽和根生长过程。分级生物测定表明，这两种生理过程的毒性顺序如下：乙酸乙酯≥正己烷＞氯仿≥正丁醇。在正己烷级分中，进行了 GC-MS 分析以表征和定量一些潜在的化感物质。鉴定出 21 种化合物，其中 3 种，樟脑、反式石竹烯和侧柏酮被定量。
